Table 1 Primers used in the study
Name | Sequence 5′ → 3′ | Description |
|---|---|---|
tl17 | TGGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGATATCAATCAGGGAAAAACGCG | acr1 forward, biobrick |
tl18 | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACCAGTGTTCGCCTGGG | acr1 reverse, biobrick |
tl33 | CGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAATTACTTCGTCACCGG | ramo forward, XbaI |
tl34 | GGCCGCTACTAGTATTATTACCAATGCTCGCCCTTG | ramo reverse, SpeI |
0030_Aar | TGGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGTTTGGTTTAATTGGCCA | aar forward, BBa0030, biobrick |
0031_Aar | TGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAACCTACTAGATGTTTGGTTTAATTGGCCA | aar forward, BBa0031, biobrick |
0032_Aar | TGGAATTCGCGGCCGCTTCTAGAGTCACACAGGAAAGTACTAGATGTTTGGTTTAATTGGCCA | aar forward, BBa0033, biobrick |
Aar_rev | CTGCAGCGGCCGCTACTAGTATTATTAAATTGCTAAAGCCAGCG | aar reverse, biobrick |