From: Bio-production of gaseous alkenes: ethylene, isoprene, isobutene
Organism | Description | Productivity | Yield (mg g−1 dcw) | Titer (g L−1) | Reference |
---|---|---|---|---|---|
Escherichia coli BL21 | ispS from I. batatas | 40 µg L−1 h−1 |  |  | [58] |
Escherichia coli BL21 | Engineered with P. alba ispS and S. cerevisiae MVA pathway | 11,083 µg L−1 h−1 |  | 0.532 | [94] |
Escherichia coli BL21 | DXS, DXR, and IDI from S. pneumoniae were overexpressed, ispA was weakened; P. alba ispS | 829 µg L−1 h−1 |  | 0.0199 | [95] |
Escherichia coli BL21 | Two component system (1) E. coli optimized for mevalonate production from sugar was used as a feedstock for (2) E. coli engineered with MVA and ispS | 230,000 µg L−1 h−1 |  | 11.0 | [96] |
Escherichia coli BL21 | ispS from P. alba, +mvaE, mvaS from E. faecalis, +mvk, PMK, MVD, IDI from S. cervisiae, + mvk from M. mazei, + pgl from E. coli | 2,000,000 µg L−1 h−1 | 850 | 60 | [97] |
Escherichia coli BL21 | ispS from P. nigra, +DXS, DXR from E. coli | Â | Â | 0.16 | [59] |
Escherichia coli BL21 | ispS from P. nigra, +DXS, DXR from B. subtilis | Â | Â | 0.31 | [59] |
Escherichia coli BL21 | ispS from P. montana, + DXS, ispG, ispH, ipi, ispE, DXR, ispD, ispF from E. coli, selection of translation initiation regions and adjustment of gene order in the superoperon | 277 µg L−1 h−1 |  | 0.005 | [22] |
Escherichia coli BL21 | ispS from P. montana, + hmgS, hmgR from E. faecalis, + atoB from E. coli, +fni, mk, pmk, pmd from S. pneumoniae, selection of translation initiation regions | 17,778 µg L−1 h−1 |  | 0.32 | [22] |
Escherichia coli BL21 | 2 mvaE, mvaS from E. faecalis, + ERG12, ERG8, ERG19, IDI from S. cerevisiae, codon-optimized ispS from P. alba, mvaS gene mutation |  |  | 6.3 | [98] |
Escherichia coli BL21 | Codon-adapted ispS from P. alba, +DXS, DXR, IDI from E. coli, adjustment of gene order in the polycistron | 2727 µg g−1 dcw h−1 |  |  | [17] |
Escherichia coli BL21 | Truncated ispS from P. alba, +DXS, DXR, IDI, pgl, fldA, ispG from E. coli, +ispH from Anabaena, +ispG system from T. elongatus | Â | Â | 8.4 | [99] |
Escherichia coli BL21 | Enhanced MEP pathway and combined with MVA pathway | 52,500 µg L−1 h−1 |  |  | [100] |
Escherichia coli MG1655 | Codon-optimized ispS from P. trichocarpa, augmented MVA pathway, and deleted genes involved in aceto-CoA byproduct formation | Â | Â | 1.832 | [101] |
Synechococcus elongatus | Fused ISPS from P. alba with IDI from S. cerevisiae | 4600 µg L−1 h−1 |  |  | [57] |
Synechocystis sp. PCC 6803 | Fused ISPS with CPCB (phyocyanin) to increase production | Â | 5.4 | Â | [102] |
Synechocystis sp. PCC 6803 | codon-optimized ispS from P. montana | 2.08 µg g−1 dcw h−1 |  |  | [103] |
Synechocystis sp. PCC 6803 | Codon-optimized ispS from P. montana | Â | 0.12 | 3.2 | [104] |
Synechocystis sp. PCC 6803 | Engineered psbA2 promoter-driven ispS and codon optimized from P. montana | 40 µg L−1 h−1 |  |  | [104] |
Synechocystis sp. PCC 6803 | Codon-optimized P. montana ispS | 2 µg L−1 h−1 |  |  | [105] |
Synechocystis sp. PCC 6803 | Codon-optimized P. montana ispS | 63 µg L−1 h−1 |  |  | [20] |
Synechocystis sp. PCC 6803 | Codon-optimized kudzu ispS under rbcL promoter | 1.16 µg L−1 h−1 A750−1 |  |  | [53] |
Bacillus subtilis | Engineered DXS, DXR |  |  | 1e−6 | [106] |
Saccharomyces cerevisiae | Multiple copies of codon-optimized ispS from P. montana | 7 µg L−1 h−1 |  | 5e−4 | [107] |
Saccharomyces cerevisiae | 2 copies of codon-optimized ispS from P. alba, +tHMG1, IDI, ACS2, ERG10 from S. cerevisiae, down-regulation of ERG20 by promoter replacement | Â | 25 | 0.037 | [108] |
Saccharomyces cerevisiae | Enhanced Gal4p supply and directed evolution of IspS | 51,388 µg L−1 h−1 |  | 3.7 | [56] |