Fig. 2

Transcriptional analysis of cells carrying T(-351)A, UTR∆A, or P10L plasmid. a Copies of WT or mutant plasmid. Cells containing WT, T(-351)A, UTR∆A, or P10L plasmid were grown in YG medium until exponential stage. Copy number was calculated relative to that of endogenous LEU2. Values represent mean ± S.D. from four parallel cultures. (*p < 0.05) b, c Relative mRNA level of Est1E expressed by WT or mutant plasmid in YG (b) or YI medium (c). mRNA level of Est1E was calculated relative to 18s rDNA and relative level of Est1E in cells containing WT plasmid at 3 h is designated as unit 1. Values represent mean ± S.D. from three parallel cultures