Fig. 4

Secretory expression of lignocellulolytic enzymes by WT or mutant plasmid. Activity of MAN330 (a), Xyn-CDBFV (c) or RuCelA (e) is indicated. Cells containing WT or mutant plasmid were grown in YG medium for 72 h, and activity in the supernatant was measured. Activity expressed by WT plasmid was designated as unit 1. Values represent mean ± S.D. from three parallel cultures. (*p < 0.05). SDS-PAGE of supernatant from representative cultures was shown in (b, d, f)