Fig. 3

Effects of FabH levels on Se7942 FA content. a Enzymatic reaction catalyzed by FabH. b Attempt to construct the Se7942 fabH-deficient mutant. Upper panel: scheme of the fabH vicinity in the wt and fabH mutant strains. Relevant genes are indicated by colored arrows, while upstream and downstream sequences flanking the deletion target (USDT and DSDT) by boxes. Primers used for PCR analysis are indicated by black arrows. Lower panel: PCR analysis of the fabH region of mutant strain MSM23. Lane 1, Hyperladder I (BioLine); lanes 2–7, MSM23 colonies; lane 8, wt Se7942 strain (WT). c FA content of wt Se7942 and the fabH merodiploid mutant (MSM23) and fabH-overexpressing derivative (MSM28). Data show the mean and + SD of at least three independent biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, by unpaired Student’s t test