Skip to main content

Table 1 Cyanobacterial strains used in this study

From: Engineering the fatty acid synthesis pathway in Synechococcus elongatus PCC 7942 improves omega-3 fatty acid production

Strain

Relevant genotype and phenotypea

Plasmids used to generate this strainb

Source or references

Synechococcus sp. PCC 7002

Wild-type strain

 

PCC

Synechococcus elongatus PCC 7942

Wild-type strain

 

PCC

GRPS1

S. elongatus PCC 7942 (rps12–R43); Smr

 

[28]

MSM17

S. elongatus PCC 7942 with PnrsB::desA–desB integrated at NS1; Spr

pMSM16

This work

MSM18

S. elongatus PCC 7942 with PnrsB::luxAB integrated at NS1; Spr

pMSM51

This work

MSM19

S. elongatus PCC 7942 with PnrsB::fabB integrated at NS1; Spr

pMSM90

This work

MSM22

S. elongatus PCC 7942 with PnrsB::riboswitch–luxAB integrated at NS1; Spr

pMSM142

This work

MSM23

GRPS1 with ΔfabH::rps12–nptI (partial deletion); Neor

pMSM182

This work

MSM24

S. elongatus PCC 7942 with Ptrc::fabD integrated at NS1; Spr

pMSM196

This work

MSM25

S. elongatus PCC 7942 with Ptrc::luxAB integrated at NS1; Spr

pMSM197

This work

MSM26

S. elongatus PCC 7942 with Ptrc::desA–desB integrated at NS1; Spr

pMSM201

This work

MSM27

S. elongatus PCC 7942 with Ptrc::fabB integrated at NS1; Spr

pMSM202

This work

MSM28

S. elongatus PCC 7942 with Ptrc::fabH integrated at NS1; Spr

pMSM228

This work

MSM29

S. elongatus PCC 7942 with Ptrc::fabF integrated at NS1; Spr

pMSM234

This work

MSM34

GRPS1 with ΔfabH::rps12–nptI; Neor

Ptrc::fabH integrated at NS1; Spr

pMSM182

pMSM228

This work

MSM35

GRPS1 ΔfabH::rps12–nptI (partial deletion); Neor

PnrsB::riboswitch–fabH integrated at NS1; Spr

pMSM182

pMSM236

This work

MSM42

GRPS1 ΔfadD::rps12- nptI; Neor

pMSM266

This work

MSM45

GRPS1

Ptrc::desA–desB integrated at NS1; Spr

Ptrc::fabF integrated at NS2; Cmr

ΔfadD::rps12–nptI; Neor

pMSM201

pMSM253

pMSM266

This work

  1. PCC Pasteur Culture Collection
  2. aSmr, streptomycin resistance; Neor, neomycin resistance; Spr, spectinomycin resistance; Cmr, chloramphenicol resistance; NS1, neutral site 1; NS2, neutral site 2. Integration of vectors into NS1 and NS2 was verified by PCR using primer pairs 102–103 and 106–108, respectively
  3. bPlasmids are listed in Additional file 3: Table S1 and, primers and details on their construction are described in Additional file 3: Table S2 and Additional file 2, respectively