Strain | Relevant genotype and phenotypea | Plasmids used to generate this strainb | Source or references |
---|---|---|---|
Synechococcus sp. PCC 7002 | Wild-type strain | Â | PCC |
Synechococcus elongatus PCC 7942 | Wild-type strain | Â | PCC |
GRPS1 | S. elongatus PCC 7942 (rps12–R43); Smr |  | [28] |
MSM17 | S. elongatus PCC 7942 with PnrsB::desA–desB integrated at NS1; Spr | pMSM16 | This work |
MSM18 | S. elongatus PCC 7942 with PnrsB::luxAB integrated at NS1; Spr | pMSM51 | This work |
MSM19 | S. elongatus PCC 7942 with PnrsB::fabB integrated at NS1; Spr | pMSM90 | This work |
MSM22 | S. elongatus PCC 7942 with PnrsB::riboswitch–luxAB integrated at NS1; Spr | pMSM142 | This work |
MSM23 | GRPS1 with ΔfabH::rps12–nptI (partial deletion); Neor | pMSM182 | This work |
MSM24 | S. elongatus PCC 7942 with Ptrc::fabD integrated at NS1; Spr | pMSM196 | This work |
MSM25 | S. elongatus PCC 7942 with Ptrc::luxAB integrated at NS1; Spr | pMSM197 | This work |
MSM26 | S. elongatus PCC 7942 with Ptrc::desA–desB integrated at NS1; Spr | pMSM201 | This work |
MSM27 | S. elongatus PCC 7942 with Ptrc::fabB integrated at NS1; Spr | pMSM202 | This work |
MSM28 | S. elongatus PCC 7942 with Ptrc::fabH integrated at NS1; Spr | pMSM228 | This work |
MSM29 | S. elongatus PCC 7942 with Ptrc::fabF integrated at NS1; Spr | pMSM234 | This work |
MSM34 | GRPS1 with ΔfabH::rps12–nptI; Neor Ptrc::fabH integrated at NS1; Spr | pMSM182 pMSM228 | This work |
MSM35 | GRPS1 ΔfabH::rps12–nptI (partial deletion); Neor PnrsB::riboswitch–fabH integrated at NS1; Spr | pMSM182 pMSM236 | This work |
MSM42 | GRPS1 ΔfadD::rps12- nptI; Neor | pMSM266 | This work |
MSM45 | GRPS1 Ptrc::desA–desB integrated at NS1; Spr Ptrc::fabF integrated at NS2; Cmr ΔfadD::rps12–nptI; Neor | pMSM201 pMSM253 pMSM266 | This work |