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Table 1 Cyanobacterial strains used in this study

From: Engineering the fatty acid synthesis pathway in Synechococcus elongatus PCC 7942 improves omega-3 fatty acid production

Strain Relevant genotype and phenotypea Plasmids used to generate this strainb Source or references
Synechococcus sp. PCC 7002 Wild-type strain   PCC
Synechococcus elongatus PCC 7942 Wild-type strain   PCC
GRPS1 S. elongatus PCC 7942 (rps12R43); Smr   [28]
MSM17 S. elongatus PCC 7942 with PnrsB::desAdesB integrated at NS1; Spr pMSM16 This work
MSM18 S. elongatus PCC 7942 with PnrsB::luxAB integrated at NS1; Spr pMSM51 This work
MSM19 S. elongatus PCC 7942 with PnrsB::fabB integrated at NS1; Spr pMSM90 This work
MSM22 S. elongatus PCC 7942 with PnrsB::riboswitchluxAB integrated at NS1; Spr pMSM142 This work
MSM23 GRPS1 with ΔfabH::rps12nptI (partial deletion); Neor pMSM182 This work
MSM24 S. elongatus PCC 7942 with Ptrc::fabD integrated at NS1; Spr pMSM196 This work
MSM25 S. elongatus PCC 7942 with Ptrc::luxAB integrated at NS1; Spr pMSM197 This work
MSM26 S. elongatus PCC 7942 with Ptrc::desAdesB integrated at NS1; Spr pMSM201 This work
MSM27 S. elongatus PCC 7942 with Ptrc::fabB integrated at NS1; Spr pMSM202 This work
MSM28 S. elongatus PCC 7942 with Ptrc::fabH integrated at NS1; Spr pMSM228 This work
MSM29 S. elongatus PCC 7942 with Ptrc::fabF integrated at NS1; Spr pMSM234 This work
MSM34 GRPS1 with ΔfabH::rps12nptI; Neor
Ptrc::fabH integrated at NS1; Spr
pMSM182
pMSM228
This work
MSM35 GRPS1 ΔfabH::rps12nptI (partial deletion); Neor
PnrsB::riboswitchfabH integrated at NS1; Spr
pMSM182
pMSM236
This work
MSM42 GRPS1 ΔfadD::rps12- nptI; Neor pMSM266 This work
MSM45 GRPS1
Ptrc::desAdesB integrated at NS1; Spr
Ptrc::fabF integrated at NS2; Cmr
ΔfadD::rps12nptI; Neor
pMSM201
pMSM253
pMSM266
This work
  1. PCC Pasteur Culture Collection
  2. aSmr, streptomycin resistance; Neor, neomycin resistance; Spr, spectinomycin resistance; Cmr, chloramphenicol resistance; NS1, neutral site 1; NS2, neutral site 2. Integration of vectors into NS1 and NS2 was verified by PCR using primer pairs 102–103 and 106–108, respectively
  3. bPlasmids are listed in Additional file 3: Table S1 and, primers and details on their construction are described in Additional file 3: Table S2 and Additional file 2, respectively