Fig. 1

Pyruvate to ethanol production in C. thermocellum using the T. saccharolyticum pyruvate to ethanol pathway; this figure is adapted from Hon et al. [4]. Metabolites and products are colored black. Native genes (Cth) and the pathways they correspondingly catalyze are colored gray; T. saccharolyticum genes (Tsc) that were previously introduced in Hon et al. [4], as well as the reactions that they catalyze, are colored green. Red arrows represent electron flux. The T. saccharolyticum pforA and ferredoxin introduced in this study are depicted in blue. The orange cross represents the pathways that were deleted in this study