Skip to main content

Table 2 List of strains and integration/deletion plasmids used in this study

From: Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production

Strains/plasmids

Organism

Description

Accession number

References or source

E. coli T7 express

Escherichia coli

fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10

 

New England Biolabs (Ipswich, MA)

M1442

T. saccharolyticum

Engineered and evolved T. saccharolyticum

SRA233073

[5]

LL1004

C. thermocellum

DSM 1313

CP002416

DSMZ

AG929

C. thermocellum

DSM1313 ∆hpt ∆clo1313_0478

SRP097241

[4]

LL1319

C. thermocellum

AG929 PClo1313_2638::adhA(Tsc)-nfnAB(Tsc)-adhEG544D (Tsc)

SRP101300

[4]

LL1565

C. thermocellum

AG929 Clo1313_2637::PpforA(Tsc)pforA(Tsc) -fd(Tsc)

SRP144031

This study

LL1391

C. thermocellum

LL1319 Clo1313_2637::PpforA(Tsc)pforA(Tsc)-fd(Tsc)

SRP141156

This study

LL1566

C. thermocellum

LL1319 Clo1313_2637::PpforA(Tsc)pforA(Tsc)

SRP144035

This study

LL1436

C. thermocellum

LL1391 ΔClo1313_0020-0023

SRP144013

This study

LL1437

C. thermocellum

LL1391 ΔClo1313_1353-1356

SRP144038

This study

LL1438

C. thermocellum

LL1436 ΔClo1313_1353-1356

SRP144037

This study

LL1567

C. thermocellum

LL1437 ΔClo1313_0020-0023

SRP144045

This study

LL1568

C. thermocellum

LL1438 ΔClo1313_0673

SRP144054

This study

LL1569

C. thermocellum

LL1568 ΔClo1313_0382-0385

SRP144051

This study

LL1570

C. thermocellum

LL1569 ΔClo1313_1615-1616

SRP144049

This study

LL1556

C. thermocellum

DSM1313 Δhpt ΔClo1313_0020-0023 Prpi: kivdLL – Ppck: ilvBNCCT – PilvD: ilvDCT

SRP144036

This study

LL1559

C. thermocellum

DSM1313 Δhpt ΔClo1313_0382-0385

SRP144040

This study

LL1563

C. thermocellum

DSM1313 Δhpt ΔClo1313_0673

 

This study

LL1564

C. thermocellum

DSM1313 Δhpt ΔClo1313_1353-1356

 

This study

LL1560

C. thermocellum

DSM1313 Δhpt ΔClo1313_1615-1616

SRP144039

This study

pJGW37

 

C. thermocellum expression plasmid

 

[19]

pSH105

 

PenolasepforA(Tsc)-fd(Tsc) integration vector

MH245114

This study

pSH106

 

PpforA(Tsc)pforA(Tsc)-fd(Tsc) integration vector

MH245115

This study

pSH107

 

PAthe_2105pforA(Tsc)-fd(Tsc) integration vector

MH245116

This study

pSH121

 

PpforA(Tsc)pforA(Tsc) integration vector

MH245113

This study

pDGO77

 

Clo1313_0020-0023 deletion vector

MH245117

This study

pDGO78

 

Clo1313_0673 deletion vector

MH245118

This study

pSH116

 

Clo1313_1353-1356 deletion vector

MH245112

This study

pSH130

 

Clo1313_0382-0385 deletion vector

MH245110

This study

pSH131

 

Clo1313_1615-1616 deletion vector

MH245111

This study