Strains/plasmids | Organism | Description | Accession number | References or source |
---|---|---|---|---|
E. coli T7 express | Escherichia coli | fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10 |  | New England Biolabs (Ipswich, MA) |
M1442 | T. saccharolyticum | Engineered and evolved T. saccharolyticum | SRA233073 | [5] |
LL1004 | C. thermocellum | DSM 1313 | CP002416 | DSMZ |
AG929 | C. thermocellum | DSM1313 ∆hpt ∆clo1313_0478 | SRP097241 | [4] |
LL1319 | C. thermocellum | AG929 PClo1313_2638::adhA(Tsc)-nfnAB(Tsc)-adhEG544D (Tsc) | SRP101300 | [4] |
LL1565 | C. thermocellum | AG929 Clo1313_2637::PpforA(Tsc)pforA(Tsc) -fd(Tsc) | SRP144031 | This study |
LL1391 | C. thermocellum | LL1319 Clo1313_2637::PpforA(Tsc)pforA(Tsc)-fd(Tsc) | SRP141156 | This study |
LL1566 | C. thermocellum | LL1319 Clo1313_2637::PpforA(Tsc)pforA(Tsc) | SRP144035 | This study |
LL1436 | C. thermocellum | LL1391 ΔClo1313_0020-0023 | SRP144013 | This study |
LL1437 | C. thermocellum | LL1391 ΔClo1313_1353-1356 | SRP144038 | This study |
LL1438 | C. thermocellum | LL1436 ΔClo1313_1353-1356 | SRP144037 | This study |
LL1567 | C. thermocellum | LL1437 ΔClo1313_0020-0023 | SRP144045 | This study |
LL1568 | C. thermocellum | LL1438 ΔClo1313_0673 | SRP144054 | This study |
LL1569 | C. thermocellum | LL1568 ΔClo1313_0382-0385 | SRP144051 | This study |
LL1570 | C. thermocellum | LL1569 ΔClo1313_1615-1616 | SRP144049 | This study |
LL1556 | C. thermocellum | DSM1313 Δhpt ΔClo1313_0020-0023 Prpi: kivdLL – Ppck: ilvBNCCT – PilvD: ilvDCT | SRP144036 | This study |
LL1559 | C. thermocellum | DSM1313 Δhpt ΔClo1313_0382-0385 | SRP144040 | This study |
LL1563 | C. thermocellum | DSM1313 Δhpt ΔClo1313_0673 |  | This study |
LL1564 | C. thermocellum | DSM1313 Δhpt ΔClo1313_1353-1356 |  | This study |
LL1560 | C. thermocellum | DSM1313 Δhpt ΔClo1313_1615-1616 | SRP144039 | This study |
pJGW37 | Â | C. thermocellum expression plasmid | Â | [19] |
pSH105 | Â | PenolasepforA(Tsc)-fd(Tsc) integration vector | MH245114 | This study |
pSH106 | Â | PpforA(Tsc)pforA(Tsc)-fd(Tsc) integration vector | MH245115 | This study |
pSH107 | Â | PAthe_2105pforA(Tsc)-fd(Tsc) integration vector | MH245116 | This study |
pSH121 | Â | PpforA(Tsc)pforA(Tsc) integration vector | MH245113 | This study |
pDGO77 | Â | Clo1313_0020-0023 deletion vector | MH245117 | This study |
pDGO78 | Â | Clo1313_0673 deletion vector | MH245118 | This study |
pSH116 | Â | Clo1313_1353-1356 deletion vector | MH245112 | This study |
pSH130 | Â | Clo1313_0382-0385 deletion vector | MH245110 | This study |
pSH131 | Â | Clo1313_1615-1616 deletion vector | MH245111 | This study |