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Table 1 Strains used in this study

From: Engineering xylose metabolism in thraustochytrid T18

Organism Strain name Relevant characteristics References
Thraustochytrid T18 Wild-type thraustochytrid. ATCC PTA-6245 [23]
XI 4 T18 transformed with pα-tub isohis. Homozygous knockout of an α-tubulin gene with ble-2A-his-xi. Zeocin-resistant This study
XI 6 T18 transformed with pα-tub isohis. Random insertion of ble-2A-his-xi. Zeocin-resistant This study
XI 8 T18 transformed with pα-tub isohis. Heterozygous knockout of an α-tubulin gene with ble-2A-his-xi. Zeocin-resistant This study
XI 16 T18 transformed with pα-tub isohis. Homozygous knockout of an α-tubulin gene with ble-2A-his-xi concatemer. Zeocin-resistant This study
XI-XK 1 XI 16 transformed with pJB47 (E. coli xylB). Zeocin and hygromycin-resistant This study
XI-XK 3 XI 16 transformed with pJB47 (E. coli xylB). Zeocin- and hygromycin-resistant This study
XI-XK 7 XI 16 transformed with pJB47 (E. coli xylB). Zeocin- and hygromycin-resistant This study
XB T18 transformed with pJB13. Homozygous knockout of an α-tubulin gene with ble-2A-xylB. Zeocin-resistant This study
E. coli W3110 F- mcrA mcrB IN(rrnD-rrnE)1 lambda. ATCC®27325 ATCC
BL21(DE3)pLysS F- ompT hsdSB(rBmB-) gal dcm (DE3) pLysS (CamR) Bioline
BL21 [pET23-XylA] AmpR, CamR. pET23-XylA (W3110 histidine-tagged- xylA) in BL21(DE3)pLysS This study
S. cerevisiae INVSC1 MATa his3Δ1 leu2 trp1-289 ura3-52/MATα his3Δ1 leu2 trp1-289 ura3-52 Invitrogen
INVSC1 [pYes2-isohis] URA. Histidine-tagged T18 xylose isomerase under the control of the GAL1 promoter This study