Table 1 Strains used in this study
Organism | Strain name | Relevant characteristics | References |
|---|---|---|---|
Thraustochytrid | T18 | Wild-type thraustochytrid. ATCC PTA-6245 | [23] |
XI 4 | T18 transformed with pα-tub isohis. Homozygous knockout of an α-tubulin gene with ble-2A-his-xi. Zeocin-resistant | This study | |
XI 6 | T18 transformed with pα-tub isohis. Random insertion of ble-2A-his-xi. Zeocin-resistant | This study | |
XI 8 | T18 transformed with pα-tub isohis. Heterozygous knockout of an α-tubulin gene with ble-2A-his-xi. Zeocin-resistant | This study | |
XI 16 | T18 transformed with pα-tub isohis. Homozygous knockout of an α-tubulin gene with ble-2A-his-xi concatemer. Zeocin-resistant | This study | |
XI-XK 1 | XI 16 transformed with pJB47 (E. coli xylB). Zeocin and hygromycin-resistant | This study | |
XI-XK 3 | XI 16 transformed with pJB47 (E. coli xylB). Zeocin- and hygromycin-resistant | This study | |
XI-XK 7 | XI 16 transformed with pJB47 (E. coli xylB). Zeocin- and hygromycin-resistant | This study | |
XB | T18 transformed with pJB13. Homozygous knockout of an α-tubulin gene with ble-2A-xylB. Zeocin-resistant | This study | |
E. coli | W3110 | F- mcrA mcrB IN(rrnD-rrnE)1 lambda. ATCC®27325 | ATCC |
BL21(DE3)pLysS | F- ompT hsdSB(rB–mB-) gal dcm (DE3) pLysS (CamR) | Bioline | |
BL21 [pET23-XylA] | AmpR, CamR. pET23-XylA (W3110 histidine-tagged- xylA) in BL21(DE3)pLysS | This study | |
S. cerevisiae | INVSC1 | MATa his3Δ1 leu2 trp1-289 ura3-52/MATα his3Δ1 leu2 trp1-289 ura3-52 | Invitrogen |
INVSC1 [pYes2-isohis] | URA. Histidine-tagged T18 xylose isomerase under the control of the GAL1 promoter | This study |