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Table 2 Gene copy number of wild-type and transformants and protein activity on xylose and xylulose

From: Engineering xylose metabolism in thraustochytrid T18

Strain Gene copy numbera % sugar used/μg/μL sugar leftb μg/μL xylulose-5-phosphatec
ble d xi aph7 Xylose Xylulose
WT 2.0 − 0.21 ± 0.28/0.55 ± 0.002 1.59 ± 0.58/0.62 ± 0.004 0.000 ± 0.004
XI 16 16.3 14.6 0.26 ± 0.29/0.55 ± 0.002 12.9 ± 0.26/0.55 ± 0.002 0.000 ± 0.001
XI-XK 1 22.7 23.2 1.8 10.2 ± 0.21/0.50 ± 0.002 42.5 ± 0.38/0.36 ± 0.002 0.035 ± 0.001
XI-XK 3 1.6 3.8 2.0 0.67 ± 0.13/0.55 ± 0.001 8.88 ± 0.67/0.58 ± 0.004 0.001 ± 0.004
XI-XK 7 26.1 26.6 23.2 26.2 ± 0.32/0.41 ± 0.002 92.1 ± 0.47/0.05 ± 0.003 0.404 ± 0.003
XB 23.6 2.0 0.14 ± 0.34/0.55 ± 0.002 97.8 ± 3.9/0.01 ± 0.025 0.619 ± 0.026
  1. N.D. not determined
  2. aGene copy numbers were determined by qPCR
  3. bCell extracts were done with set amounts of xylose (0.55 μg/μL) and xylulose (0.63 μg/μL) and the amount of sugar detected was determined by HPLC. The amount of sugar converted or detected following incubation with 0.08 μg/μL total protein extract is shown
  4. cXylulose-5-phosphate produced with 0.08 μg/μL total protein was calculated by deducting the total amount of xylose and xylulose detected in these reactions from the known starting amount of xylulose
  5. dble values in the XB transformant indicate the gene copies of ble-2A-xylB, whereas, in the XI and XI-XK transformants, it indicates gene copies of ble-2A-his-xi