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Table 3 Differential expression of key genes related to fatty acid biosynthesis

From: Transcriptome and gene expression analysis of docosahexaenoic acid producer Schizochytrium sp. under different oxygen supply conditions

Gene

Fatty acid biosynthesis

Δ-8 desaturase

Δ-6 desaturase

Δ-12 desaturase

Elongase

FAS

ORFA

ORFB

ORFC

RPKM

 Normal-oxygen

161.4

0.9

50.56

1.45

4.8

2822.5

199.96

167.98

987.24

348.6

591.44

 High-oxygen

142.5

1.96

46.69

10.02

8.98

7046.7

128.16

666.83

1851.4

610.65

1328.4

  1. The RPKM method is able to eliminate the influence of different gene length and sequencing discrepancy on the calculation of gene expression. Therefore, the calculated gene expression can be directly used for comparing the difference of gene expression among samples
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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