Skip to main content

Table 3 Subset of genes with significant differential transcript levels in D5A during nitrogen-deprived condition (5 mM, − N) compared to nitrogen-repleted condition (35 mM, + N) in time points of T1, T2, and T3, as well as D5A compared to BY4741

From: Oleaginicity of the yeast strain Saccharomyces cerevisiae D5A

Name

D5A (− N/+ N) (Log2)

D5A/BY4741 (Log2)

Function

FA/TAG synthesis

OLE1

1.2

4.5

Delta(9) fatty acid desaturase

ELO3

1.5

0.2

Elongase

PIS1

1.1

− 0.3

Phosphatidylinositol synthase

POX1

− 1.4

2.4

Fatty-acyl coenzyme A oxidase

FAA1

− 1.4

0.8

Long-chain fatty-acyl-CoA synthetase

N metabolism

GLT1

1.6

1.0

NAD(+)-dependent glutamate synthase (GOGAT)

GLN1

1.9

0.00

Glutamine synthetase (GS)

GDH1

1.4

− 0.3

NADP(+)-dependent glutamate dehydrogenase

GDH2

0.6

0.5

NAD(+)-dependent glutamate dehydrogenase

AA metabolism

ARO8

3.4

− 1.5

Aromatic aminotransferase I

LEU2

2.4

6.9

Beta-isopropylmalate dehydrogenase (IMDH)

CHA1

2.7

− 3.6

Catabolic l-serine (l-threonine) deaminase

BAT1

3.3

− 2.5

Mitochondrial branched-chain amino acid (BCAA) aminotransferase

ASN1

2.4

− 3.1

Asparagine synthetase

EMP

HXK2

2.9

− 0.3

Hexokinase isoenzyme 2

TDH1

− 3.8

1.4

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

HXK1

− 3.2

1.4

Hexokinase isoenzyme 1

GLK1

− 3.0

1.9

Glucokinase

GPM1

− 2.1

− 0.3

Tetrameric phosphoglycerate mutase

FBP1

− 2.1

1.6

Fructose-1,6-bisphosphatase

PYK2

− 2.1

− 0.6

Pyruvate kinase

PGK1

− 1.9

− 0.1

3-Phosphoglycerate kinase

PPP

TKL1

2.4

− 0.1

Transketolase

SOL3

1.4

− 326

6-Phosphogluconolactonase

GND1

1.1

0.3

6-Phosphogluconate dehydrogenase (decarboxylating)

TKL2

− 3.3

3.0

Transketolase

TAL1

− 2.1

0.3

Transaldolase

ZWF1

− 1.7

− 1.0

Glucose-6-phosphate dehydrogenase (G6PD)

TCA cycle

PYC1

− 2.0

0.3

Pyruvate carboxylase isoform

CIT1

− 3.3

1.1

Citrate synthase

CIT3

− 2.6

− 0.3

Dual specificity mitochondrial citrate and methylcitrate synthase

ACO1

− 1.2

2.5

Aconitase

SDH1

− 1.5

1.8

Flavoprotein subunit of succinate dehydrogenase

MDH1

− 2.3

0.8

Mitochondrial malate dehydrogenase

SDH2

− 1.6

1.9

Iron–sulfur protein subunit of succinate dehydrogenase

FUM1

− 1.0

1.3

Fumarase

KGD1

− 1.5

1.0

mitochondrial α-ketoglutarate dehydrogenase complex subunit

KGD2

− 1.1

1.7

Dihydrolipoyl transsuccinylase

ICL1

− 1.4

1.4

Isocitrate lyase

Other

YAT1

− 4.1

0.5

Outer mitochondrial carnitine acetyltransferase

CIT2

− 4.9

− 0.4

Citrate synthase

ICL1

− 1.4

1.4

Isocitrate lyase

ACS1

− 3.5

2.1

Acetyl-coA synthetase isoform

PDC6

1.5

0.7

Minor isoform of pyruvate decarboxylase

ADH4

− 3.3

4.0

Alcohol dehydrogenase isoenzyme type IV

ALD6

− 1.2

1.3

Cytosolic aldehyde dehydrogenase

GPP1

1.2

− 0.5

Constitutively expressed dl-Glycerol-3-phosphate phosphatase

GPP2

1.5

− 0.7

dl-Glycerol-3-phosphate phosphatase involved in glycerol biosynthesis

  1. All transcriptional differences are shown as the mean of duplicate log2-based values