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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producer Clostridium saccharobutylicum

Fig. 4

General diagram representing gene replacement via allelic exchange at the target gene. a C. saccharobutylicum NCP262 genomic regions surrounding CLSA_RS14125 (hsdR2). The deletion vector pChN1, containing approximately 1 kbp of upstream and downstream sequences of hsdR2 and the codBA operon from C. ljungdahlii. b Counterselection strategy with the 5-FC/codBA system resulting in a marker-less deletion mutant lacking CLSA_RS14125 (hsdR2) between the two flanking regions

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