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Table 1 Transconjugation efficiencies with unmethylated pMTL84151 donor plasmid

From: Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producer Clostridium saccharobutylicum

C. saccharobutylicum strain Conjugation efficiency with unmethylated pMTL84151
WT 0
ΔhsdR1 3.2 ± 0.7 × 10−4
Ch1 6.8 ± 1.1 × 10−4
Ch2 3.7 ± 1.5 × 10−3
  1. Transconjugation efficiencies were calculated as the ratio of colonies on colistin plates with and without thiamphenicol. Mean values and standard deviations from three independent experiments are given