Skip to main content

Table 1 Transconjugation efficiencies with unmethylated pMTL84151 donor plasmid

From: Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producer Clostridium saccharobutylicum

C. saccharobutylicum strain

Conjugation efficiency with unmethylated pMTL84151

WT

0

ΔhsdR1

3.2 ± 0.7 × 10−4

Ch1

6.8 ± 1.1 × 10−4

Ch2

3.7 ± 1.5 × 10−3

  1. Transconjugation efficiencies were calculated as the ratio of colonies on colistin plates with and without thiamphenicol. Mean values and standard deviations from three independent experiments are given
Back to article page

Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

Contact us