Table 3 Bacterial strains and plasmids used in this study

Bacterial strains

 E. coli

  TOP10

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG

Invitrogen

  DH10B

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL λ-

Invitrogen

  CA434

HB101 carrying the IncPb conjugative plasmid, R702, Kan^R

Purdy et al. [47]

 C. saccharobutylicum

  NCP262

Wild type

DSMZ^a

  hsdR1::int

CLSA_RS02150::intron, ermB

Lesiak et al. [4]

  ΔhsdR1

Δ CLSA_RS02150

This study

  ΔhsdR1, hsdR2::pChN1

Δ CLSA_RS02150, CLSA_RS14125 integration of pChN1

This study

  Ch1

Δ CLSA_RS02150 Δ CLSA_RS14125

This study

  Ch2

Δ CLSA_RS02150 Δ CLSA_RS14125 Δ CLSA_RS04425

This study

  Ch1 ΔxylB

Δ CLSA_RS02150 Δ CLSA_RS14125 Δ CLSA_RS15825

This study

  Ch1 ΔptbΔbuk

Δ CLSA_RS02150 Δ CLSA_RS14125

Δ CLSA_RS01285 Δ CLSA_RS01290

This study

 Plasmids

  pJL2

Derived from pACYC184, hsdMSII_T7, Tc^R

Lesiak et al. [4]

  pMTL84151

pCD6, Cm^R

Heap et al. [46]

  pKVM4

oripE194ts, oripBR322, pclpB, bla, ermC, oriT, traJ, codBA from E. coli

Kostner et al. [35]

  pJIR750

Cm^R, lacZ, oripMB1, oripIP404

Bannam and Rood [51]

  pCN3

oripE194ts, oripBR322, Cm^R, oriT, traJ, codBA from E. coli

This study

  pCN6

Δ CLSA_RS02150, oripBR322, Cm^R, oriT, traJ, codBA from E. coli

This study

  pCN8

Δ CLSA_RS14125, oripBR322, Cm^R, oriT, traJ, codBA from E. coli

This study

  pChN

oripBR322, Cm^R, oriT, traJ, codBA gene from C. ljungdahlii

This study

  pChN1

Δ CLSA_RS14125, Cm^R, codBA gene from C. ljungdahlii

This study

  pChN2

Δ CLSA_RS04425, Cm^R, codBA gene from C. ljungdahlii

This study

  pChN3

Δ CLSA_RS15825, Cm^R, codBA gene from C. ljungdahlii

This study

  pChN4

Δ CLSA_RS01285 Δ CLSA_RS01290, Cm^R, codBA gene from C. ljungdahlii

This study