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Table 3 Bacterial strains and plasmids used in this study

From: Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producer Clostridium saccharobutylicum

Strain or plasmid Relevant characteristics References
Bacterial strains
 E. coli
  TOP10 F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
  DH10B F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara, leu)7697 araD139 galU galK nupG rpsL λ- Invitrogen
  CA434 HB101 carrying the IncPb conjugative plasmid, R702, KanR Purdy et al. [47]
 C. saccharobutylicum
  NCP262 Wild type DSMZa
  hsdR1::int CLSA_RS02150::intron, ermB Lesiak et al. [4]
  ΔhsdR1 Δ CLSA_RS02150 This study
  ΔhsdR1, hsdR2::pChN1 Δ CLSA_RS02150, CLSA_RS14125 integration of pChN1 This study
  Ch1 Δ CLSA_RS02150 Δ CLSA_RS14125 This study
  Ch2 Δ CLSA_RS02150 Δ CLSA_RS14125 Δ CLSA_RS04425 This study
  Ch1 ΔxylB Δ CLSA_RS02150 Δ CLSA_RS14125 Δ CLSA_RS15825 This study
  Ch1 ΔptbΔbuk Δ CLSA_RS02150 Δ CLSA_RS14125
Δ CLSA_RS01285 Δ CLSA_RS01290
This study
 Plasmids
  pJL2 Derived from pACYC184, hsdMSIIT7, TcR Lesiak et al. [4]
  pMTL84151 pCD6, CmR Heap et al. [46]
  pKVM4 oripE194ts, oripBR322, pclpB, bla, ermC, oriT, traJ, codBA from E. coli Kostner et al. [35]
  pJIR750 CmR, lacZ, oripMB1, oripIP404 Bannam and Rood [51]
  pCN3 oripE194ts, oripBR322, CmR, oriT, traJ, codBA from E. coli This study
  pCN6 Δ CLSA_RS02150, oripBR322, CmR, oriT, traJ, codBA from E. coli This study
  pCN8 Δ CLSA_RS14125, oripBR322, CmR, oriT, traJ, codBA from E. coli This study
  pChN oripBR322, CmR, oriT, traJ, codBA gene from C. ljungdahlii This study
  pChN1 Δ CLSA_RS14125, CmR, codBA gene from C. ljungdahlii This study
  pChN2 Δ CLSA_RS04425, CmR, codBA gene from C. ljungdahlii This study
  pChN3 Δ CLSA_RS15825, CmR, codBA gene from C. ljungdahlii This study
  pChN4 Δ CLSA_RS01285 Δ CLSA_RS01290, CmR, codBA gene from C. ljungdahlii This study
  1. aDSMZ Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures