Table 4 Oligonucleotides used for PCR amplification
Primer name | Oligonucleotides sequence | Function |
|---|---|---|
pCN3_V_F | GAAAACTTTTTGCGTGTGACAG | pCN3 backbone |
pCN3_V_R | CTGTCAGACCAAGTTTAC | |
catp_FpJIR_IV | GTAAACTTGGTCTGACAGACCGTATTTCTACGATGTTT | catP gene from pJIR750 |
catp_RpJIR_IV | CTGTCACACGCAAAAAGTTTTCTTTCGGCAAGTGTTCAAG | |
FlankA_F6_IV | GATTACAAACGTTGAAGAAGGAAGGAACTGGTCCAGAAG | hsdR1 upstream |
HsdR1_A_Fu_R | CATTTCTTTAGTTCCCTTCTTAATATTTTCCCCCCTACATTC | |
HsdR1_B_Fu_F | GAATGTAGGGGGGAAAATATTAAGAAGGGAACTAAAGAAATG | hsdR1 downstream |
FlankB_R6_IV | CTTGAACACTTGCCGAAAAATGGAGGATTTGCCAATA | |
pCN6_V_F | CTTCTTCAACGTTTGTAATC | pCN6/pCN8 backbone |
pCN6_V_R | TTTCGGCAAGTGTTCAAG | |
HsdR1_check_F | GCAGGAGAAAGGATATGG | hsdR1 wild type or mutant |
HsdR1_check_R | CGATACTCCTGCATATGG | |
check_preR | ACACAACCGGCACAAACC | check integration |
Check_catp_F | AACTATTTATCAATTCCTGCAATTCGTTTAC | catP gene on the deletion vector |
Check_catp_R | ATGGTATTTGAAAAAATTGATAAAAATAGTTG | |
HsdR2_A_F_IV | CTTGAACACTTGCCGAAAGTGTTAGGTTTAAAGAATAC | hsdR2 upstream |
HsdR2_A_Fu_R | GAATAATTAGGAGGGGATTTGATAATAGTTTAATGGCTATTG | |
HsdR2_B_Fu_F | CAATAGCCATTAAACTATTATCAAATCCCCTCCTAATTATTC | hsdR2 downstream |
HsdR2_B_R_IV | GATTACAAACGTTGAAGAAGAAGACTGGGATCGATAGC | |
pCLcodBA_F_IV | CTACTTAATTGTGTGTAAGATAAAGAAGAAGACTGGGATCGAT | pChN1 backbone |
pCLcodBA_R_IV | CATCAATTACCTCCTAAATTAATAATTAGCTAATTTTCGTTTAATTAT | |
CLcodBA_F2 | AATTATTAATTTAGGAGGTAATTGATG | codBA gene from C. ljungdahlii |
CLcodBA_R2 | TTATCTTACACACAATTAAGTAG | |
HsdR2_check_F | GGTGGTTCTACAGCAATCTC | hsdR2 wild type or mutant |
HsdR2_check_R | GCTAAGGACGTTGGATTAGC | |
pChN_backbone_F | TACTTAATTGTGTGTAAGATAAGTTTCGGCAAGTGTTCAAGAAG | pChN backbone |
pChN_backbone_R | CTTATCTTACACACAATTAAGTAGAAGAAC | |
HsdR3_A_F_IV | GTTCTTCTACTTAATTGTGTGTAAGATAAGTGTCTATTCAAGTGCTGTGG | hsdR3 upstream |
HsdR3_A_R_IV | GAAATACAGGGGGTGTTAAC GCTTACAAGACCACAACTAG | |
HsdR3_B_F_IV | CTAGTTGTGGTCTTGTAAGC GTTAACACCCCCTGTATTTC | hsdR3 downstream |
HsdR3_B_R_IV | CTTCTTGAACACTTGCCGAAA GCTGCAATAGCAAAATATCG | |
pChN_V_F | TTTCGGCAAGTGTTCAAGAAG | pChN2/pChN3/pChN4 backbone |
pChN_V_R | CTTATCTTACACACAATTAAGTAGAAGAAC | |
HsdR3_check_F | TGCTAAAGTATCGCGGTTGTC | hsdR3 wild type or mutant |
HsdR3_check_R | AGCCGTTCTGAAATTGAACTG | |
codBA_CL_R | TATGTGGATGGGGAAGAG | Check integration |
xylB_A_F_IV | ACTTAATTGTGTGTAAGATAAG CTAATCCATCCGTTATTG | xylB upstream |
xylB_A_fu_R2_IV | GTTTATTGATGAGGTATT CTTATCCTAGAATTAAAG | |
xylB_B_fu_F2_IV | CTTTAATTCTAGGATAAG AATACCTCATCAATAAAC | xylB downstream |
xylB_B_R_IV | CTTGAACACTTGCCGAAA TTATTAGATGCTTCTTAG | |
xylB_check_F | ATTCTCCCGATGAATTATTG | xylB wild type or mutant |
xylB_check_R | TCCTTCGTTCAATTAAATC | |
PTB_F_IV | TTAATTGTGTGTAAGATAAG ATAAAGCGCCAGTACAGC | ptb upstream |
PTB_R2_fu_IV | CTTTAGCTTCTTCTTCTCCA TCCTTTAATCTTGATAG | |
BUK_R_IV | CTTGAACACTTGCCGAAA ACCTAGTACTCCCTGTTC | buk downstream |
BUK_F2_fu_IV | CTATCAAGATTAAAGGA TGGAGAAGAAGAAGCTAAAG | |
PTB_check_F3 | CGGCATTAGTTGTAACTG | Ptb–buk wild type or mutant |
BUK_check_R2 | GCTCCACTTGCATTCATC |