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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: A versatile system for fast screening and isolation of Trichoderma reesei cellulase hyperproducers based on DsRed and fluorescence-assisted cell sorting

Fig. 2

Isolation of cellulase hyperproducers from ace3-transformants by FACS using the intracellularly expressing DsRed as an indicator. A Microscopic observation of the hyphae of T. reesei expressing intracellular DsRed and its parental strain SUS2. Mycelia were harvested from T. reesei cultivated at 28 °C for 24 h in MM-lactose/sophorose. B Flow cytometry sorting of germlings with the brightest DsRed signal (top 0.1%) from ace3-transformants. The area in box was the gating strategy used to delineate the brightest DsRed signal cell populations. C Preliminary assessment of endoglucanase producing and protein secretion ability of ace3-transformants in shake flask fermentation. D The cellulose-hydrolyzing halos formed by isolated representative ace3-transformants. Different letters (a, b, and c) mean that there are significant difference between the colony diameters (p < 0.05). E, F Representative ace3-transformants isolated in FACS produced more endoglucanase (E) and secreted more proteins (F)

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