Fig. 2
From: Current advance in bioconversion of methanol to chemicals
Strategies to enhance the metabolic flux from methanol to metabolites. Methanol is first oxidized to formaldehyde by peroxisomal enzyme alcohol oxidase, which is assimilated via the xylulose 5-phosphate pathway (XuMP) (red lines). Formaldehyde dissimilation pathway is blocked (purple line). The regeneration of xylulose 5-phosphate is improved by overexpressed genes of pentose phosphate pathway (green lines). Enzymes: Aod, alcohol oxidase; Cta, catalase; Das, dihydroxyacetone synthase; Dak, dihydroxyacetone kinase; Tkt, transketolase; Fba, fructose-bisphosphate aldolase; Pfk, phosphofructokinase; Fbp, fructose bisphosphatase; Pgi, phosphoglucose isomerase. Metabolites, DHA, dihydroxyacetone; DHAP, dihydroxyacetone phosphate; GAP, glyceraldehyde 3-phosphate; F6P, fructose 6-phosphate; FBP, fructose 1,6-bisphosphate; Xu5P, xylulose 5-phosphate; E4P, erythrose 4-phosphate; G6P, glucose 6-phosphate