Table 1 Various of chemicals production using native or synthetic methylotrophs

PHAs

PHB

40–52.9 g/L

0.09–0.12 g/g

M. extorquens ATCC 55366

[16]

9.5 g/L

0.16 g/g

M. extorquens DSMZ 1340

[18]

136 g/L

0.18 g/g

Pseudomonas sp. K

[90]

PHB (8000 –10,000 kDa)

7.04 g/L

0.17 g/g

M. halotolerans C2

[17]

PHB-co-HV

~ 1 g/L

0.17 g/g

Methylobacterium sp. strain GW2

[21]

PHB-co-HV

2.34 g/L

–

Methylocystis sp. WRRC1

[91]

Amino acids

l-Glutamate

38.8 g/L

~ 0.13 g/g

M. glycogenes

[25]

55 g/L

0.36 g/g

Bacillus methanolicus MGA3

[27, 28]

l-Threonine

11 g/L

–

M. glycogenes AL119

[25]

l-Lysine

8 g/L

–

Introduction of the dapA gene encoding dihydrodipicolinate synthase into M. glycogenes AL119

[26]

35 g/L

–

B. methanolicus MGA3 mutant 13A52-8A66

[27]

11.3 g/L

–

Expressed l-lysine/l-arginine exporter (LysE) in M. methylotrophus

[30]

l-Serine

54.5 g/L

8.3% from methanol, 39.3% from glycine

M. extorquens

[31]

65 mg/mL

0.62 g/g

Methylobacterium sp. MN43

[32]

Organic acids

Pyruvate

0.26 g/L

0.25 g/g

The methanol metabolic pathways originating from P. pastoris were constructed into the chromosome of S. cerevisiae

[2]

Mesaconic

70 mg/L

0.0175 g/g

Expressed thioesterase yciA in M. extorquens

[44]

Methylsuccinic acid

60 mg/L

0.015 g/g

Expressed thioesterase yciA in M. extorquens

[44]

Fine chemicals

Mevalonate

2.67 g/L

0.085 g/g

Biosensor-assisted transcriptional regulator engineering used for M. extorquens

[54]

α-Humulene

1.65 g/L

0.031 g/g

Expressed α-humulene synthase and farnesyl pyrophosphate synthase in M. extorquens

[56]

Naringenin

3.5 mg/L

4.7% ¹³C-labeling in naringenin

The pathways for methanol assimilation and naringenin synthesis were introduced into E. coli

[60]

Monacolin J

593.9 mg/L

0.35 mg/g

The biosynthetic pathways for monacolin J and lovastatin were assembled into P. pastoris

[65]

Lovastatin

250.8 mg/L

0.15 mg/g

The biosynthetic pathways for monacolin J and lovastatin were assembled into P. pastoris

[65]

Other chemicals

Cadaverine

11.3 g/L

–

Expressed lysine decarboxylase in B. methanolicus

[67]

1.5 g/L

–

The pathways for methanol assimilation and lysine decarboxylase were introduced into C. glutamicum

[64]

γ-Aminobutyric acid

9 g/L

–

Expressed glutamate decarboxylase in B. methanolicus MGA3

[77]

Proteins

Green fluorescent protein (GFP)

4 g/L

0.3 g/g

Expressed green fluorescent protein in M. extorquens

[82]

Enterocin P

155 ng/mL

–

Expressed the Enterocin P structural gene in M. extorquens

[84]

Cry1Aa

1.26 mg/L

9 mg/g DCW, 4.5% of total protein

Engineered M. extorquens

[85]