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Table 1 Various of chemicals production using native or synthetic methylotrophs

From: Current advance in bioconversion of methanol to chemicals

Substance class Product Concentration Yield Organism Reference
PHAs PHB 40–52.9 g/L 0.09–0.12 g/g M. extorquens ATCC 55366 [16]
  9.5 g/L 0.16 g/g M. extorquens DSMZ 1340 [18]
  136 g/L 0.18 g/g Pseudomonas sp. K [90]
PHB (8000 –10,000 kDa) 7.04 g/L 0.17 g/g M. halotolerans C2 [17]
PHB-co-HV ~ 1 g/L 0.17 g/g Methylobacterium sp. strain GW2 [21]
  PHB-co-HV 2.34 g/L Methylocystis sp. WRRC1 [91]
Amino acids l-Glutamate 38.8 g/L ~ 0.13 g/g M. glycogenes [25]
55 g/L 0.36 g/g Bacillus methanolicus MGA3 [27, 28]
l-Threonine 11 g/L M. glycogenes AL119 [25]
l-Lysine 8 g/L Introduction of the dapA gene encoding dihydrodipicolinate synthase into M. glycogenes AL119 [26]
35 g/L B. methanolicus MGA3 mutant 13A52-8A66 [27]
  11.3 g/L Expressed l-lysine/l-arginine exporter (LysE) in M. methylotrophus [30]
l-Serine 54.5 g/L 8.3% from methanol, 39.3% from glycine M. extorquens [31]
65 mg/mL 0.62 g/g Methylobacterium sp. MN43 [32]
Organic acids Pyruvate 0.26 g/L 0.25 g/g The methanol metabolic pathways originating from P. pastoris were constructed into the chromosome of S. cerevisiae [2]
Mesaconic 70 mg/L 0.0175 g/g Expressed thioesterase yciA in M. extorquens [44]
Methylsuccinic acid 60 mg/L 0.015 g/g Expressed thioesterase yciA in M. extorquens [44]
Fine chemicals Mevalonate 2.67 g/L 0.085 g/g Biosensor-assisted transcriptional regulator engineering used for M. extorquens [54]
α-Humulene 1.65 g/L 0.031 g/g Expressed α-humulene synthase and farnesyl pyrophosphate synthase in M. extorquens [56]
Naringenin 3.5 mg/L 4.7% 13C-labeling in naringenin The pathways for methanol assimilation and naringenin synthesis were introduced into E. coli [60]
Monacolin J 593.9 mg/L 0.35 mg/g The biosynthetic pathways for monacolin J and lovastatin were assembled into P. pastoris [65]
Lovastatin 250.8 mg/L 0.15 mg/g The biosynthetic pathways for monacolin J and lovastatin were assembled into P. pastoris [65]
Other chemicals Cadaverine 11.3 g/L Expressed lysine decarboxylase in B. methanolicus [67]
1.5 g/L The pathways for methanol assimilation and lysine decarboxylase were introduced into C. glutamicum [64]
γ-Aminobutyric acid 9 g/L Expressed glutamate decarboxylase in B. methanolicus MGA3 [77]
Proteins Green fluorescent protein (GFP) 4 g/L 0.3 g/g Expressed green fluorescent protein in M. extorquens [82]
Enterocin P 155 ng/mL Expressed the Enterocin P structural gene in M. extorquens [84]
Cry1Aa 1.26 mg/L 9 mg/g DCW, 4.5% of total protein Engineered M. extorquens [85]