Fig. 2

Effect of the glycosyltransferase deletion on protein glycosylation and CelA stability. a, b, and c are SDS-PAGE gels and the protein loadings are the same. Molecular weight standards (lane 1), JWCB001 (wild type) ECP (lane 2), JWCB018 (ΔpyrF parent strain) ECP (lane 3), JWCB143 (glycosyltransferase deletion) ECP (lane 4), JWCB001 (wild type) ICP (lane 5), JWCB018 (ΔpyrF parent strain) ICP (lane 6), and JWCB143 (glycosyltransferase deletion) ICP (lane 7). a Stained with glycoprotein stain. Lane 8 is a glycosylation positive standard. b The same gel counterstained with RAPIDstain to visualize protein. c The same protein fractions run on an SDS-PAGE gel stained with Coomassie Brilliant Blue without prior glycoprotein stain. d Western Blot. molecular weight standards (lane 1), JWCB001 ECP (lane 2), JWCB018 ECP (lane 3), JWCB143 ECP (lane 4), JWCB029 ECP (lane 5), JWCB001 ICP (lane 6), JWCB018 ICP (lane 7), JWCB143 ICP (lane 8), and JWCB029 ICP (lane 9). CBM3c was the primary antibody with an HRP-linked secondary antibody, visualized by chemiluminescence