Table 1 Strains/plasmid used in this study

C. bescii

 JWCB001

DSMZ6725 wild type (ura⁺/5-FOA^S)

DSMZ

 JWCB018

ΔpyrFA ldh::ISCbe4 Δcbe1 (ura⁻/5-FOA^R)

[4, 44]

 JWCB029

ΔpyrFA ldh::ISCbe4 Δcbe1 ΔcelA (ura⁻/5-FOA^R)

[10]

 JWCB143

ΔpyrFA ldh::ISCbe4 Δcbe1 Δcbes1864 (ura⁻/5-FOA^R)

This study

 JWCB160

JWCB143-containing pJRW013 (ura⁺/5-FOA^S)

This study

E. coli

 JW563

DH5α-containing pJRW012 (Apramycin^R)

This study

 JW626

DH5α-containing pJRW013 (Apramycin^R)

This study

Plasmids

 pDCW173

E. coli/C. bescii shuttle vector (Apramycin^R)

[14]

 pJGW003

C. bescii integration vector (Apramycin^R)

[42]

 pJYW022

Expression vector for Cbes_1867 (Apramycin^R)

This study

 pJRW012

Cbes1864 deletion vector (Apramycin^R)

This study

 pJRW013

Expression vector for Cbes_1864 (Apramycin^R)

This study