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Table 1 Strains and plasmids in this study

From: Optimizing the localization of astaxanthin enzymes for improved productivity

Strains/plasmids Relevant characteristics Source/notes
Strains
 CAR010 CAR005 [27], mRSL-4::ispG, mRSL-14::ispH Unpublished
 CAR003 CAR010, ∆crtX Unpublished
 CAR025 CAR005 [27], ispG-mRSL-4, ispH-mRSL-14, replacing the promoter of crtEYIB with trc promoter [22]
Plasmids
 pSC101 Low copy plasmid Unpublished
 pACYC184-M cat; replace tet with lacI and Ptrc of pTrc99A-M [27]
 pACYC184M2-Pm46 cat; kan; expression vector; replace lacI and Ptrc of pAYCA184-M with FRT-Km-FRT::M1-46 Unpublished
 pSC102 Low copy plasmid, ori and repA from pSC101, M1-46 promoter, cat from pACYC184-M2-Pm46 Unpublished
 pYL002 crtW in pSC102 Unpublished
 pYL501 crtW and crtZ in pSC102 Unpublished
 pGlpF-CrtW glpF fused with crtW in pSC102 This study
 pCrtZ crtZ in pSC102 This study
 pGlpF-CrtZ glpF fused with crtZ in pSC102 This study
 pGlpF-CrtW/GlpF-CrtZ glpF fused with crtW and glpF fused with crtZ in pSC102 This study
 pCrtW-CrtZ crtW fused with crtZ in pSC102 This study
 pGlpF-CrtW-CrtZ glpF fused with crtW and crtZ in pSC102 This study
 pPhCCD1 PhCCD1 in pACYC184M2-Pm46 This study
 pGlpF-PhCCD1 glpF fused with PhCCD1 in pACYC184M2-Pm46 This study
 pMBP-PhCCD1 MBP fused with PhCCD1 in pACYC184M2-Pm46 This study
 pSPompA-PhCCD1 The signal peptide sequence of ompA fused with PhCCD1 in pACYC184M2-Pm46 This study
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