Table 1 Strains and plasmids in this study
From: Optimizing the localization of astaxanthin enzymes for improved productivity
Strains/plasmids | Relevant characteristics | Source/notes |
|---|---|---|
Strains | ||
CAR010 | CAR005 [27], mRSL-4::ispG, mRSL-14::ispH | Unpublished |
CAR003 | CAR010, ∆crtX | Unpublished |
CAR025 | CAR005 [27], ispG-mRSL-4, ispH-mRSL-14, replacing the promoter of crtEYIB with trc promoter | [22] |
Plasmids | ||
pSC101 | Low copy plasmid | Unpublished |
pACYC184-M | cat; replace tet with lacI and Ptrc of pTrc99A-M | [27] |
pACYC184M2-Pm46 | cat; kan; expression vector; replace lacI and Ptrc of pAYCA184-M with FRT-Km-FRT::M1-46 | Unpublished |
pSC102 | Low copy plasmid, ori and repA from pSC101, M1-46 promoter, cat from pACYC184-M2-Pm46 | Unpublished |
pYL002 | crtW in pSC102 | Unpublished |
pYL501 | crtW and crtZ in pSC102 | Unpublished |
pGlpF-CrtW | glpF fused with crtW in pSC102 | This study |
pCrtZ | crtZ in pSC102 | This study |
pGlpF-CrtZ | glpF fused with crtZ in pSC102 | This study |
pGlpF-CrtW/GlpF-CrtZ | glpF fused with crtW and glpF fused with crtZ in pSC102 | This study |
pCrtW-CrtZ | crtW fused with crtZ in pSC102 | This study |
pGlpF-CrtW-CrtZ | glpF fused with crtW and crtZ in pSC102 | This study |
pPhCCD1 | PhCCD1 in pACYC184M2-Pm46 | This study |
pGlpF-PhCCD1 | glpF fused with PhCCD1 in pACYC184M2-Pm46 | This study |
pMBP-PhCCD1 | MBP fused with PhCCD1 in pACYC184M2-Pm46 | This study |
pSPompA-PhCCD1 | The signal peptide sequence of ompA fused with PhCCD1 in pACYC184M2-Pm46 | This study |