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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

Fig. 3

Multi-locus integration. a Editing efficiencies of gfpmut3a at three different loci. b Flow cytometry analysis of the expression of GFP in strains OP012 (OP001ΔOpLEU2::gfpmut3a), OP015 (OP001ΔOpURA3::gfpmut3a) and OP018 (OP001ΔOpHIS3::gfpmut3a). c The sketch map of simultaneous integrations of TAL, 4CL and STS genes at OpURA3, OpHIS3 and OpLEU2 loci, respectively. d Biosynthetic pathway of resveratrol by integrating TAL, 4CL and STS genes (in blue). e Editing efficiencies of multi-locus integrations with and without the expression of targeting gRNAs. f HPLC analysis of resveratrol. g Cell growth and resveratrol productions of the mutant strain OP021 (OP001ΔOpHIS3::4CL ΔOpURA3::TAL ΔOpLEU2::STS) and the wild-type strain

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