Fig. 4
From: Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
Multi-copy integration of gfpmut3a at rDNA repeats of O. polymorpha. a The Cas9 delivery vector pWYE3219. The expression of Cas9 was driven by the inducible promoter POpMOX. b A schematic illustration of integration of multi-copy integration at rDNA repeats. c Precise integration site of exogenous gene at rDNA cluster in O. polymorpha. d Integration efficiencies of gfpmut3a at rDNA repeats with and without the expression of targeting gRNA. Error bars indicate standard deviations of three biological repeats. e Copy numbers of gfpmut3a in eight randomly selected colonies. f Stability of multi-copy integration of gfpmut3a at rDNA repeats in the mutant OP025 (OP001rDNA::gfpmut3a)