Fig. 5
From: Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
The application of multi-copy integration method in O. polymorpha. a Copy numbers of the fusion expression cassette PScTEF1-TAL-PScTPI1-4CL-PScTEF2-STS integrated at rDNA cluster. b Resveratrol productions of colonies harboring different copy numbers of the fusion expression cassette PScTEF1-TAL-PScTPI1-4CL-PScTEF2-STS. c Copy numbers of cadA integrated at rDNA cluster. d HPLC analysis of cadaverine in O. polymorpha. e Cell growth and cadaverine productions of the wild-type strain OP001 and the mutant OP028 (OP001rDNA::cadA). f Copy numbers of gene HSA integrated at rDNA cluster in eight randomly selected colonies. g HSA productions of the mutant strain OP031(OP001rDNA::HSA) by shake flask fermentation at different fermentation time