Fig. 6
From: Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
Multi-copy integration of gfpmut3a at rDNA cluster in S. cerevisiae. a Precise integration site of gfpmut3a at rDNA cluster. b Integration efficiencies of gfpmut3a at the rDNA repeats with and without the expression of targeting gRNA. c Copy numbers of gfpmut3a in eight randomly selected colonies. d Flow cytometry analysis of the expression of gfpmut3a in eight randomly selected colonies. e Stability of multi-copy integration at rDNA repeats in the mutant SC007 (SC001 rDNA::gfpmut3a)