Table 1 Description of E. coli base strains, plasmids and gene clusters used in this study
From: Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
Plasmid reference | Plasmid name | Relevant genotype | References | |
|---|---|---|---|---|
JBEI-2704 | placUV5-MevT-MBIS | p15A, CmR | [44] | |
JBEI-2872 | pgadE-MevT-MBIS | p15A, CmR | [25] | |
JBEI-15857 | pTrc99a-EIZS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15862 | pTrc99a-coEIZS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15867 | pTrc99a-coPentS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15865 | pTrc99a-coMrTPS2 | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15856 | pTrc99a-his-EIZS | ColE1(pBR322) ori, AmpR, his tag | This study | |
JBEI-15855 | pTrc99a-his-coEIZS | ColE1(pBR322) ori, AmpR, his tag | This study | |
JBEI-15854 | pTrc99a-his-coPentS | ColE1(pBR322) ori, AmpR, his tag | This study | |
JBEI-15853 | pTrc99a-his-coMrTPS2 | ColE1(pBR322) ori, AmpR, his tag | This study | |
JBEI-15849 | prstA-coEIZS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15863 | prstA-coPentS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15864 | prstA-coMrTPS2 | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15866 | pBbE7a-coEIZS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15858 | pBbE7a-coPentS | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15859 | pBbE7a-coMrTPS2 | ColE1(pBR322) ori, AmpR | This study | |
JBEI-15861 | pRSLeu2d-coPentS | 2μ ori, pBR322 ori, Leu2d | This study |
Strain name | Description | References | |
|---|---|---|---|
CL1601 | JBEI-2704 + JBEI-15862 | This study | |
CL1602 | JBEI-2704 + JBEI-15849 | This study | |
CL1603 | JBEI-2872 + JBEI-15849 | This study | |
CL1604 | JBEI-2872 + JBEI-15862 | This study | |
CL1605 | JBEI-2704 + JBEI-15866 | This study | |
CL1606 | JBEI-2872 + JBEI-15866 | This Study | |
CL1607 | JBEI-2704 + JBEI-15867 | This study | |
CL1608 | JBEI-2704 + JBEI-15863 | This Study | |
CL1609 | JBEI-2872 + JBEI-15863 | This study | |
CL1610 | JBEI-2872 + JBEI-15867 | This study | |
CL1611 | JBEI-2704 + JBEI-15858 | This study | |
CL1612 | JBEI-2872 + JBEI-15858 | This study | |
CL1613 | JBEI-2704 + JBEI-15865 | This study | |
CL1614 | JBEI-2704 + JBEI-15864 | This study | |
CL1615 | JBEI-2872 + JBEI-15864 | This study | |
CL1616 | JBEI-2872 + JBEI-15865 | This study | |
CL1617 | JBEI-2704 + JBEI-15859 | This study | |
CL1618 | JBEI-2872 + JBEI-15859 | This study | |
CL1619 | S. cerevisiae EPY300 harboring JBEI-15861 | This study |