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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression in Talaromyces pinophilus

Fig. 6

EMSA revealing interactions between TpRfx1 DNA-binding domain and the promoter regions of amylase genes. The indicated amounts of purified TpRfx1190-367 were mixed with about 150 ng of FAM-labeled EMSA probes. EMSA probes lacking the FAM label and the promoter region of β-tubulin gene were used for competitive EMSA and as a negative control, respectively. BSA alone and TRX-His-S from E. coli cells containing the empty vector pET-32a(+) were used as controls

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