Fig. 6
EMSA revealing interactions between TpRfx1 DNA-binding domain and the promoter regions of amylase genes. The indicated amounts of purified TpRfx1190-367 were mixed with about 150 ng of FAM-labeled EMSA probes. EMSA probes lacking the FAM label and the promoter region of β-tubulin gene were used for competitive EMSA and as a negative control, respectively. BSA alone and TRX-His-S from E. coli cells containing the empty vector pET-32a(+) were used as controls