Reduced use of phosphorus and water in sequential dark fermentation and anaerobic digestion of wheat straw and the application of ensiled steam-pretreated lucerne as a macronutrient provider in anaerobic digestion

Table 3 Substrate and products concentrations of osmotolerant Caldicellulosiruptor cultivation on hydrolysates and defined media EB1 and modified DSM 640

Component (g/L)	CH-1		WSH-1		EB-1		Modified DSM 640
Component (g/L)	Before DF	After DF	Before DF	After DF	Before DF	After DF	Before DF	After DF
Microbial biomass		1.13 ± 0.00		1.34 ± 0.01		0.56 ± 0.04		0.72 ± 0.02
Glucose	14.52	10.81 ± 0.45	18.82 ± 0.05	14.84 ± 0.17	18.82	18.17 ± 0.56	18.82	18.47 ± 0.47
Xylose	6.25	1.55 ± 0.18	8.19 ± 0.24	1.914 ± 0.28	8.2	3.50 ± 0.21	8.2	2.84 ± 0.93
Arabinose	0.42	ND	0.58 ± 0.01	ND	0.59	ND	0.59	ND
Acetate	1.79	7.74 ± 0.36	1.60 ± 0.56	7.20 ± 0.56	ND	2.80 ± 0.38	ND	3.15 ± 0.45
Lactate	0.39	0.61 ± 0.075	ND	0.18 ± 0.11	ND	0.27 ± 0.07	ND	0.20
Ethanol	0.06	0.12 ± 0.000	ND	0.82 ± 0.02	ND	0.15 ± 0.01	ND	0.11 ± 0.02
Propionate	0.095	0.18 ± 0.030	ND	ND	ND	ND	ND	ND

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