Fig. 4
From: Determinants of selection in yeast evolved by genome shuffling
Mutations in NRG1 and GSH1 are the main determinants of hydrolysate tolerance. Multiple linear regression (a) on the genotypes of segregants of an SSL-tolerant mutant identifies mutations nrg1-G137T and gsh1-T(-73)A as the strongest predictors of high tolerance. This prediction is confirmed in point mutants (b), with both mutations conferring increased tolerance to SSL. The nrg1-G137T mutation also increases tolerance to acetic acid and hydrogen peroxide in haploids. Bars in the graphs report area under the growth curve for the indicated mutants recorded in the presence (solid bars) and absence (striped bars) of inhibitors. Error bars represent ± 1 standard deviation