Fig. 1

Settling of stationary phase-grown cultures (a) and growth curves (b) of M. luteus trpE16 (wt), the olefin-overproducing strain M. luteus ope and of the olefin gene cluster knockout strain M. luteus ΔoleABCD:kan. The strains were grown in rich medium (LB) for 40 h with shaking at 30 °C and afterwards left undisturbed for 4 h. The growth curve measurements were performed with 4 independent cultures, the growth rate constants (r) were estimated by fitting the absorbance data points (black dots) to the standard form of the logistic equation (red line), using the drc package in the R programming language [18]