A global analysis of gene expression in Fibrobacter succinogenes S85 grown on cellulose and soluble sugars at different growth rates

Table 1 Culture summary statistics

Substrate	Target D (h⁻¹)	Actual D (h⁻¹)	Succinate	Acetate	Formate	Total soluble sugar	SC20 consumed (g L⁻¹)
Cellulose	0.018	0.018	15.56 ± 2.40	7.24 ± 1.00	0.52 ± 0.39	1.31 ± 0.08	1.83
Cellulose	0.047	0.047	7.05 ± 0.97	3.11 ± 0.52	0.21 ± 0.38	0.56 ± 0.06	0.31
Cellulose	0.068	0.068	9.56 ± 1.14	2.92 ± 0.47	0.09 ± 0.15	0.72 ± 0.05	0.74
Glucose	0.018	0.019	12.70 ± 0.41	5.01 ± 0.15	0.17 ± 0.03	0.81 ± 0.04
Glucose	0.047	0.047	10.09 ± 0.49	4.39 ± 1.40	0.69 ± 0.46	1.45 ± 0.14
Glucose	0.068	0.067	10.80 ± 0.35	3.86 ± 0.15	0.31 ± 0.08	0.98 ± 0.14
Glucose	0.200	0.198	11.25 ± 1.68	3.79 ± 1.90	0.94 ± 0.55	0.84 ± 0.03
Cellobiose	0.018	0.016	13.33 ± 0.99	4.66 ± 0.36	ND	0.97 ± 0.05
Cellobiose	0.047	0.047	10.54 ± 0.44	3.36 ± 0.13	0.21 ± 0.11	1.19 ± 0.05
Cellobiose	0.068	0.068	10.69 ± 0.36	3.58 ± 0.08	0.44 ± 0.09	1.01 ± 0.17
Cellobiose	0.200	0.193	10.03 ± 0.49	3.32 ± 0.16	0.50 ± 0.08	0.82 ± 0.07
ANOVA*		Growth rate (GR)	0.070	0.007
		Substrate (S)	0.518	0.095
		GR × S	0.002	1.18 × 10⁻⁶

Values of fermentation products and total soluble sugars are mean ± 1 standard deviation of three biological replicates
D dilution rate, ND not detected
*Results reported for ANOVA of the two factors and interaction are P values

ISSN: 2731-3654