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Table 1 Steady-state kinetic parameters of aldol reactions to DHA catalyzed by RhaD and FucA

From: Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies

 

Product

Donor

Acceptor

Conversion (%)

Vmax (U/mg)

KM (mM)

kcat (s−1)

103 × kcat/KM (s−1 mM−1)

RhaD

l-Fructose

DHAP

l-GAL

92a

4.3 ± 0.5

40.8 ± 5.8

20.5 ± 2.5

500 ± 64

Dendroketose

DHAP

DHA

73

0.86 ± 0.24

23.4 ± 9.69

4.1 ± 0.52

180 ± 40

FucA

l-Tagatose

DHAP

l-GAL

NT

1.5 ± 0.3

54.1 ± 3.6

5.0 ± 0.53

92 ± 13

Dendroketose

DHAP

DHA

17

0.32 ± 0.13

309.8 ± 45.2

0.45 ± 0.13

1.4 ± 0.5

  1. NT not test
  2. aThis result has been shown in previous paper [38]
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