Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies

Table 2 The key information of enzymes used in multi-enzyme system

Enzyme	Resource	Uniprot/genebank	Specific activities (U/mg)	References
l-Rhamnulose-1-phosphate aldolase (RhaD)	Escherichia coli	P32169	0.27	This study
Fructose-1-phosphatase (YqaB)	Escherichia coli	P77475	0.6	[38]
Formolase (FLS)^a	Pseudomonas fluorescens	P51853^a	0.09	This study
DHA kinase (DhaK)	Citrobacter freundii	P45510	22	[37]
Polyphosphate kinase (PPK)	Rhodobacter sphaeroides	AWD23073.1	18.2	This study

^aThe enzyme FLS was a mutant of benzaldehyde lyase (BAL) (Uniprot P51853) at A28I, A394G, G419N, and A480W substitution

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