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Table 3 Synthesis of dendroketose using different carbon sources

From: Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies

Strains

Medium

Substrates

Production (g/L)

Yield (mol/mol)

WT(pXRTY/pEFDK)a

CGXII

Glycerol and DHA

5.6 ± 0.4

0.68 ± 0.03

WT(pXRTY/pEFDK)b

CGXII

Glycerol

0.8 ± 0.1

0.05 ± 0.01

SY6(pXRTY/pEFDK)b

CGXII

Glycerol

1.2 ± 0.2

0.46 ± 0.02

SY6(pXRTY/pEFDK)b

BHI

Glycerol

6.4 ± 0.4

0.45 ± 0.02

WT(pXRTYH)c

CGXII

Glucose

ND

–

SY6(pXRTYH)c

CGXII

Glucose

36.3 ± 1.3

0.46 ± 0.02

SY6(pXFucTYH)c

CGXII

Glucose

8.2 ± 0.6

0.12 ± 0.02

  1. ND not detected
  2. aThe initial concentration of glycerol and DHA was 220 mM and 110 mM, respectively
  3. bThe initial concentration of glycerol was 220 mM
  4. cThe initial concentration of glucose was 220 mM. The fermentation was carried out for 48 h. Mean and standard deviation were calculated based on triplicate experiments