Table 4 Strains and plasmids used in this study
Strains and plasmids | Genotype and properties | Source or references |
|---|---|---|
Strain | ||
 E. coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Novagen |
 E. coli BL21 (DE3) | F-ompT hsdSB (Rb-mB-) gal (λ c I 857 ind1 Sam7 nin5 lacUV5 T7gene1) dcm (DE3) | Novagen |
 C. glutamicum | Wild-type strain | ATCC13032 |
 SY6 | Gene tpi knock-out in C. glutamicum 13032 | [40] |
 WT(pXRTYH) | Wide-type containing plasmid pXRTYH | This study |
 SY6(pXRTYH) | Strain SY6 containing plasmid pXRTYH | This study |
 SY6(pXFucTYH) | Strain SY6 containing plasmid pXFucTYH | This study |
 WT(pXRTY/pEFDK) | Wide-type containing plasmid pXRTY and pEFDK | [46] |
 SY6(pXRTY/pEFDK) | Strain SY6 containing plasmid pXRTY and pEFDK | This study |
Plasmid | ||
 pET21a(+) | Expression vector, ApR | Invitrogen |
 pET21-RhaD | pET21a(+) derivative carrying gene of rhaD | [40] |
 pET21-FucA | pET21a(+) derivative carrying gene of fucA | This study |
 pET21-FLS | pET21a(+) derivative carrying gene of FLS | [34] |
 pET21-YqaB | pET21a(+) derivative carrying gene of yqaB | [38] |
 pET21-DhaK | pET21a(+) derivative carrying gene of DhaK | This study |
 pXRTY | pXMJ19 derivative carrying gene of rhaD and yqaB | [40] |
 pEFDK | pECXK99E derivative carrying gene of glpF, dhaD and dhaK | [46] |
 pXRTYH | pXRTY derivative carrying gene of hdpA | This study |
 pXFucTYH | pXFucTY derivative carrying gene of hdpA | This study |