Fig. 8

The products of MtLPMO9 action on PASC in the presence of HT-A-WS lignin after incorporating a lignin pre-incubation step in buffer, centrifuge the reaction mixture and using either the supernatant or the precipitate as a reducing agent for the enzyme activity. a MtLPMO9 and lignin added simultaneously on PASC and incubated for 24 h, b lignin was pre-incubated in buffer for 24 h, then the supernatant was added to PASC together with MtLPMO9 and incubated for another 24 h, c lignin was pre-incubated in buffer for 24 h, then the precipitant was added to PASC together with MtLPMO9 and incubated for another 24 h, d PASC was pre-incubated with lignin for 24 h, then MtLPMO9 was added and incubated for another 24 h, e lignin and PASC were incubated for 24 h