Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp. CGMCC 5087 and heterologous overproduction in Escherichia coli

Liu, Changqing; Zhang, Kai; Cao, Wenyan; Zhang, Ge; Chen, Guoqiang; Yang, Haiyan; Wang, Qian; Liu, Haobao; Xian, Mo; Zhang, Haibo

doi:10.1186/s13068-018-1297-3

Biotechnology for Biofuels and Bioproducts

Table 1 Plasmids and strains used in this study

From: Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp. CGMCC 5087 and heterologous overproduction in Escherichia coli

Name	Relevant characteristics	References
Plasmids
pETDuet-1	ColE1(pBR322) ori; Amp^r; P_T7	Novagen
pETDuet-KDC0498	ColE1(pBR322) ori; Amp^r; P_T7-kdc0498	This work
pETDuet-KDC0505	ColE1(pBR322) ori; Amp^r; P_T7-kdc0505	This work
pETDuet-KDC1244	ColE1(pBR322) ori; Amp^r; P_T7-kdc1244	This work
pETDuet-KDC1476	ColE1(pBR322) ori; Amp^r; P_T7-kdc1476	This work
pETDuet-KDC3074	ColE1(pBR322) ori; Amp^r; P_T7-kdc3074	This work
pETDuet-KDC3075	ColE1(pBR322) ori; Amp^r; P_T7-kdc3075	This work
pETDuet-KDC3076	ColE1(pBR322) ori; Amp^r; P_T7-kdc3076	This work
pETDuet-KDC3652	ColE1(pBR322) ori; Amp^r; P_T7-kdc3652	This work
pETDuet-KDC4427	ColE1(pBR322) ori; Amp^r; P_T7-kdc4427
pETDuet-KDC4472	ColE1(pBR322) ori; Amp^r; P_T7-kdc4472	This work
pETDuet-Aro10	ColE1(pBR322) ori; Amp^r; P_T7-ARO10	This work
pETDuet-aroFcoli–pheAcoli	ColE1(pBR322) ori; Amp^r; P_T7-aroFcoli–pheAcoli	Reference [37]
pETDuet-aroFcoli-pheA3272	ColE1(pBR322) ori; Amp^r; P_T7-aroFcoli-pheA3272	This work
pETDuet-aroFcoli-pheA3270	ColE1(pBR322) ori; Amp^r; P_T7-aroFcoli-pheA3270	This work
pETDuet-aroG1710-pheAcoli	ColE1(pBR322) ori; Amp^r; P_T7-aroG1710-pheAcoli	This work
pETDuet-aroG1710-pheA3272	ColE1(pBR322) ori; Amp^r; P_T7-aroG1710-pheA3272	This work
pETDuet-aroG1710-pheA3270	ColE1(pBR322) ori; Amp^r; P_T7-aroG1710-pheA3270	This work
pETDuet-aroH2193-pheAcoli	ColE1(pBR322) ori; Amp^r; P_T7-aroH2193-pheAcoli	This work
pETDuet-aroH2193-pheA3272	ColE1(pBR322) ori; Amp^r; P_T7-aroH2193-pheA3272	This work
pETDuet-aroH2193-pheA3270	ColE1(pBR322) ori; Amp^r; P_T7-aroH2193-pheA3270	This work
pETDuet-aroF3269-pheAcoli	ColE1(pBR322) ori; Amp^r; P_T7-aroF3269-pheAcoli	This work
pETDuet-aroF3269-pheA3272	ColE1(pBR322) ori; Amp^r; P_T7-aroF3269-pheA3272	This work
pETDuet-aroF3269-pheA3270	ColE1(pBR322) ori; Amp^r; P_T7-aroF3269-pheA3270	This work
pTrcHis2B-AtPAL-FDC1-ppsA–tktA	pBR322 ori; Amp^r; P_Trc-AtPAL-FDC1-ppsA–tktA	Reference [37]
pETDuet-aroFcoli–pheAcoli–ppsA–tktA	ColE1(pBR322) ori; Amp^r; P_T7-aroFcoli–pheAcoli–ppsA–tktA	This work
pACYCDuet-1	P15A origin; Cm^R; P_T7	Novagen
pACYCduet-ADH2	P15A origin; Cm^R; P_T7-ADH2	This work
pACYCduet-adh4428	P15A origin; Cm^R; P_T7-adh4428	This work
pACYCduet-kdc4427–adh4428	P15A origin; Cm^R; P_T7-kdc4427–adh4428	This work
Strains
E. coli BL21(DE3)	E. coli B dcm ompT hsdS(rB⁻mB⁻) gal	Takara
E. coli DH5α	deoR, recA1, endA1, hsdR17(rk⁻, mk⁺), phoA, supE44, λ⁻, thi⁻¹, gyrA96, relA1	Invitrogen
E. coli BL01	E. coli BL21(DE3) harboring pETDuet-kdc0428 and pACYCDuet-ADH2	This work
E. coli BL02	E. coli BL21(DE3) harboring pETDuet-kdc0505 and pACYCDuet-ADH2	This work
E. coli BL03	E. coli BL21(DE3) harboring pETDuet-kdc1244 and pACYCDuet-Adh2	This work
E. coli BL04	E. coli BL21(DE3) harboring pETDuet-kdc1476 and pACYCDuet-ADH2	This work
E. coli BL05	E. coli BL21(DE3) harboring pETDuet-kdc3074 and pACYCDuet-ADH2	This work
E. coli BL06	E. coli BL21(DE3) harboring pETDuet-kdc3075 and pACYCDuet-ADH2	This work
E. coli BL07	E. coli BL21(DE3) harboring pETDuet-kdc3076 and pACYCDuet-Adh2	This work
E. coli BL08	E. coli BL21(DE3) harboring pETDuet-kdc3653 and pACYCDuet-ADH2	This work
E. coli BL09	E. coli BL21(DE3) harboring pETDuet-kdc4427 and pACYCDuet-ADH2	This work
E. coli BL10	E. coli BL21(DE3) harboring pETDuet-ARO10 and pACYCDuet-Adh4428	This work
E. coli BL11	E. coli BL21(DE3) harboring pETDuet-ARO10 and pACYCDuet-ADH2	This work
E. coli BL12	E. coli BL21(DE3) pETDuet-kdc4427 and pACYCDuet-adh4428	This work
E. coli BL13	E. coli BL21(DE3) harboring pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP1	E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheAcoli and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP2	E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheA3272 and harboring pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP3	E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheA3270 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP4	E. coli BL21(DE3) harboring pETDuet-aroG1710-pheAcoli and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP5	E. coli BL21(DE3) harboring pETDuet-aroG1710–pheA3272 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP6	E. coli BL21(DE3) harboring pETDuet-aroG1710–pheA3270 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP7	E. coli BL21(DE3) harboring pETDuet-aroH2193-pheAcoli and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP8	E. coli BL21(DE3) harboring pETDuet-aroH2193–pheA3272 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP9	E. coli BL21(DE3) harboring pETDuet-aroH2193–pheA3270 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP10	E. coli BL21(DE3) harboring pETDuet-aroF3269–pheAcoli and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP11	E. coli BL21(DE3) harboring pETDuet-aroF3269–pheA3272 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13AP12	E. coli BL21(DE3) harboring pETDuet-aroF3269–pheA3270 and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13TP1	E. coli BL21(DE3) harboring pETDuet-tktA–ppsA and pACYCDuet-kdc4427–adh4428	This work
E. coli BL13TP2	E. coli BL21(DE3) harboring pETDuet-tktA–ppsA–aroFcoli–pheAcoli and pACYCDuet-kdc4427–adh4428	This work
E. coli LCQ-1	E. coli BL21(DE3) harboring pETDuet-kdc4427	This work
E. coli LCQ-2	E. coli BL21(DE3) harboring pETDuet-ARO10	This work
E. coli LCQ-3	E. coli BL21(DE3) harboring pETDuet-adh4428	This work
E. coli LCQ-4	E. coli BL21(DE3) harboring pETDuet-ADH2	This work

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