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Table 1 Plasmids and strains used in this study

From: Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp. CGMCC 5087 and heterologous overproduction in Escherichia coli

Name Relevant characteristics References
Plasmids
 pETDuet-1 ColE1(pBR322) ori; Ampr; PT7 Novagen
 pETDuet-KDC0498 ColE1(pBR322) ori; Ampr; PT7-kdc0498 This work
 pETDuet-KDC0505 ColE1(pBR322) ori; Ampr; PT7-kdc0505 This work
 pETDuet-KDC1244 ColE1(pBR322) ori; Ampr; PT7-kdc1244 This work
 pETDuet-KDC1476 ColE1(pBR322) ori; Ampr; PT7-kdc1476 This work
 pETDuet-KDC3074 ColE1(pBR322) ori; Ampr; PT7-kdc3074 This work
 pETDuet-KDC3075 ColE1(pBR322) ori; Ampr; PT7-kdc3075 This work
 pETDuet-KDC3076 ColE1(pBR322) ori; Ampr; PT7-kdc3076 This work
 pETDuet-KDC3652 ColE1(pBR322) ori; Ampr; PT7-kdc3652 This work
 pETDuet-KDC4427 ColE1(pBR322) ori; Ampr; PT7-kdc4427  
 pETDuet-KDC4472 ColE1(pBR322) ori; Ampr; PT7-kdc4472 This work
 pETDuet-Aro10 ColE1(pBR322) ori; Ampr; PT7-ARO10 This work
 pETDuet-aroFcolipheAcoli ColE1(pBR322) ori; Ampr; PT7-aroFcolipheAcoli Reference [37]
 pETDuet-aroFcoli-pheA3272 ColE1(pBR322) ori; Ampr; PT7-aroFcoli-pheA3272 This work
 pETDuet-aroFcoli-pheA3270 ColE1(pBR322) ori; Ampr; PT7-aroFcoli-pheA3270 This work
 pETDuet-aroG1710-pheAcoli ColE1(pBR322) ori; Ampr; PT7-aroG1710-pheAcoli This work
 pETDuet-aroG1710-pheA3272 ColE1(pBR322) ori; Ampr; PT7-aroG1710-pheA3272 This work
 pETDuet-aroG1710-pheA3270 ColE1(pBR322) ori; Ampr; PT7-aroG1710-pheA3270 This work
 pETDuet-aroH2193-pheAcoli ColE1(pBR322) ori; Ampr; PT7-aroH2193-pheAcoli This work
 pETDuet-aroH2193-pheA3272 ColE1(pBR322) ori; Ampr; PT7-aroH2193-pheA3272 This work
 pETDuet-aroH2193-pheA3270 ColE1(pBR322) ori; Ampr; PT7-aroH2193-pheA3270 This work
 pETDuet-aroF3269-pheAcoli ColE1(pBR322) ori; Ampr; PT7-aroF3269-pheAcoli This work
 pETDuet-aroF3269-pheA3272 ColE1(pBR322) ori; Ampr; PT7-aroF3269-pheA3272 This work
 pETDuet-aroF3269-pheA3270 ColE1(pBR322) ori; Ampr; PT7-aroF3269-pheA3270 This work
 pTrcHis2B-AtPAL-FDC1-ppsAtktA pBR322 ori; Ampr; PTrc-AtPAL-FDC1-ppsAtktA Reference [37]
 pETDuet-aroFcolipheAcolippsAtktA ColE1(pBR322) ori; Ampr; PT7-aroFcolipheAcolippsAtktA This work
 pACYCDuet-1 P15A origin; CmR; PT7 Novagen
 pACYCduet-ADH2 P15A origin; CmR; PT7-ADH2 This work
 pACYCduet-adh4428 P15A origin; CmR; PT7-adh4428 This work
 pACYCduet-kdc4427adh4428 P15A origin; CmR; PT7-kdc4427adh4428 This work
Strains
 E. coli BL21(DE3) E. coli B dcm ompT hsdS(rBmB) gal Takara
 E. coli DH5α deoR, recA1, endA1, hsdR17(rk, mk+), phoA, supE44, λ, thi−1, gyrA96, relA1 Invitrogen
 E. coli BL01 E. coli BL21(DE3) harboring pETDuet-kdc0428 and pACYCDuet-ADH2 This work
 E. coli BL02 E. coli BL21(DE3) harboring pETDuet-kdc0505 and pACYCDuet-ADH2 This work
 E. coli BL03 E. coli BL21(DE3) harboring pETDuet-kdc1244 and pACYCDuet-Adh2 This work
 E. coli BL04 E. coli BL21(DE3) harboring pETDuet-kdc1476 and pACYCDuet-ADH2 This work
 E. coli BL05 E. coli BL21(DE3) harboring pETDuet-kdc3074 and pACYCDuet-ADH2 This work
 E. coli BL06 E. coli BL21(DE3) harboring pETDuet-kdc3075 and pACYCDuet-ADH2 This work
 E. coli BL07 E. coli BL21(DE3) harboring pETDuet-kdc3076 and pACYCDuet-Adh2 This work
 E. coli BL08 E. coli BL21(DE3) harboring pETDuet-kdc3653 and pACYCDuet-ADH2 This work
 E. coli BL09 E. coli BL21(DE3) harboring pETDuet-kdc4427 and pACYCDuet-ADH2 This work
 E. coli BL10 E. coli BL21(DE3) harboring pETDuet-ARO10 and pACYCDuet-Adh4428 This work
 E. coli BL11 E. coli BL21(DE3) harboring pETDuet-ARO10 and pACYCDuet-ADH2 This work
 E. coli BL12 E. coli BL21(DE3) pETDuet-kdc4427 and pACYCDuet-adh4428 This work
 E. coli BL13 E. coli BL21(DE3) harboring pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP1 E. coli BL21(DE3) harboring pETDuet-aroFcolipheAcoli and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP2 E. coli BL21(DE3) harboring pETDuet-aroFcolipheA3272 and harboring pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP3 E. coli BL21(DE3) harboring pETDuet-aroFcoli–pheA3270 and pACYCDuet-kdc4427–adh4428 This work
 E. coli BL13AP4 E. coli BL21(DE3) harboring pETDuet-aroG1710-pheAcoli and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP5 E. coli BL21(DE3) harboring pETDuet-aroG1710pheA3272 and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP6 E. coli BL21(DE3) harboring pETDuet-aroG1710pheA3270 and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP7 E. coli BL21(DE3) harboring pETDuet-aroH2193-pheAcoli and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP8 E. coli BL21(DE3) harboring pETDuet-aroH2193pheA3272 and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP9 E. coli BL21(DE3) harboring pETDuet-aroH2193pheA3270 and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP10 E. coli BL21(DE3) harboring pETDuet-aroF3269pheAcoli and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP11 E. coli BL21(DE3) harboring pETDuet-aroF3269pheA3272 and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13AP12 E. coli BL21(DE3) harboring pETDuet-aroF3269pheA3270 and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13TP1 E. coli BL21(DE3) harboring pETDuet-tktAppsA and pACYCDuet-kdc4427adh4428 This work
 E. coli BL13TP2 E. coli BL21(DE3) harboring pETDuet-tktAppsAaroFcolipheAcoli and pACYCDuet-kdc4427adh4428 This work
 E. coli LCQ-1 E. coli BL21(DE3) harboring pETDuet-kdc4427 This work
 E. coli LCQ-2 E. coli BL21(DE3) harboring pETDuet-ARO10 This work
 E. coli LCQ-3 E. coli BL21(DE3) harboring pETDuet-adh4428 This work
 E. coli LCQ-4 E. coli BL21(DE3) harboring pETDuet-ADH2 This work