Table 2 Primers used in this study
Primers | Nucleotide sequencea |
|---|---|
pETDuet-kdc0498-F | CGCggatcctATGAATCACATGAATAAAC |
pETDuet-kdc0498-R | CCCaagcttATGCGCTTGTAAGACC |
pETDuet-kdc0505-F | CGCggatcctGTGGCAAAAGTGGAACCCG |
pETDuet-kdc0505-R | cgaattcTCATGCCATCCCCTTCGC |
pETDuet-kdc1244-F | CGCggatcctATGAAACGACTCATTGTTGG |
pETDuet-kdc1244-R | CCCaagcttCAGGCCCCTTGCCAGCG |
pETDuet-kdc1476-F | CGCggatcctATGAACACCTTCGACAAAC |
pETDuet-kdc1476-R | CCCaagcttACAGGTTATCTGGAAAG |
pETDuet-kdc3074-F | CGCggatcctATGACGGGGGCAACGGGC |
pETDuet-kdc3074-R | CCCaagcttCAAATGCCATCTTTATTCTC |
pETDuet-kdc3075-F | CGCggatcctATGGCATTTGATGATTTGAG |
pETDuet-kdc3075-R | cagagctcTTATTGACGTGCTGCCAGC |
pETDuet-kdc3076-F | CGCggatcctATGATTTGTCCACGTTGTGC |
pETDuet-kdc3076-R | CCCaagcttACAGCAGCGGCGGGATTG |
pETDuet-kdc3652-F | CGCggatcctATGATTAAATCATTAACGTCC |
pETDuet-kdc3652-R | cgaattcTCAATCTGCGGAAATGGC |
pETDuet-kdc4427-F | CGCggatcctATGCGTACCCCATACTGC |
pETDuet-kdc4427-R | ATAAGAATgcggccgcCAGGCGCTATTGCGCGC |
pETDuet-ARO10-F | CGCggatcctATGGCACCTGTTACAATTG |
pETDuet-ARO10-R | cagagctCTATTTTTTATTTCTTTTAAGTG |
pACYC-ADH2-F | catcagatctccatcaccatcatcaccacATGTCTATTCCAGAAACTC |
pACYC-ADH2-R | cggggtaccTTATTTAGAAGTGTCAAC |
pACYC-adh4428-F | GAagatctCATGGGTTATCAGCCGGACA |
pACYC-adh4428-R | CCGctcgagTTATTTTGAGCTGTTCAGGATTG |
pheA3272-F | GA agatctc ATGACACCGGAAAACCCGTTAC |
pheA3272-R | CCGctcgag TTAGGCCGGGTCAACCG |
pheA3270-F | GA agatctc ATGGTTGCTGAATTGACCG |
pheA3270-R | CCGctcgag TTACTGGCGACTGTCATTTG |
aroG1710-F | GCggatcctATGAATTATCAGAACGACGATTTACGCAT |
aroG1710-R | ATAAGAATgcggccgcTTAGCCGCGACGCGCTTTTA |
aroH2193-F | GCggatcctATGAATAAAACCGATGAACTCCG |
aroH2193-R | ATAAGAATgcggccgcTTAGAAGCGAGAATCAACCG |
aroF3269-F | GCg gat cct TTGAGGAAAACAACTATCGCA |
aroF3269-R | ATAAGAATgcggccgc TTAAGCCAGACGCGTCG |
ppsA–tktA-F | TCCGctcgagTTAAGGAGGTATATATTAATGTCCAACAATGGCTCGT |
ppsA–tktA-R | TCCGcctaggTTACAGCAGTTCTTTTGCTTTCG |