Fig. 5

TLC analysis of the hydrolysis products of colloidal chitin and chitooligosaccharide (NAG₃–NAG₆) generated by purified MtCh509. The reaction mixture (25 μL), which contained 0.3 μg of purified MtCh509, 50 mM citrate buffer (pH 5.0), and substrates, was incubated at 55 °C for various times (0, 1, 15, 30, 60, 180, 360, 540, or 720 min). A Colloidal chitin was used as a substrate; B NAG₃ was used as a substrate; C NAG₄ was used as a substrate; D NAG₅ was used as a substrate; E NAG₆ was used as a substrate; F NAG₃ was used as a substrate and reacted using 0.6 μg of purified MtCh509. Lane STD is a standard mixture of NAG₁–NAG₆. Lane C is a substrate control without purified MtCh509