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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221

Fig. 6

HPLC analysis of the hydrolysate of colloidal chitin generated by purified MtCh509. a Standard peaks of NAG1–NAG6. b Hydrolysate of colloidal chitin generated by purified MtCh509 (black arrow, buffer; red arrow, NAG1; white arrow, NAG3). The reaction mixture (500 μL), which contained 6 μg of purified MtCh509, 50 mM citrate buffer (pH 5.0), and 1% colloidal chitin, was incubated at 55 °C for 12 h

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