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Table 1 Effect of different metal ions and chemical reagents on MtCh509

From: Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221

Substances Relative activity (%)
1 mM 5 mM 10 mM
Control 100 100 100
Ni2+ 100 ± 5.8 69 ± 6.9 32 ± 1.8
Cu2+ 99 ± 5.6 91 ± 4.9 6 ± 1.8
Mg2+ 93 ± 5.2 67 ± 6.9 66 ± 3.9
Li+ 86 ± 6.3 85 ± 1.6 67 ± 7.4
Ba2+ 81 ± 1.0 82 ± 0.4 73 ± 1.3
K+ 82 ± 2.8 84 ± 0.9 50 ± 3.4
Zn2+ 87 ± 0.8 77 ± 0.0 20 ± 0.6
Mn2+ 66 ± 3.2 49 ± 0.9 50 ± 1.1
Ca2+ 59 ± 2.5 51 ± 1.6 51 ± 6.0
Co2+ 58 ± 2.6 52 ± 1.6 25 ± 2.0
Cs2+ 40 ± 2.0 37 ± 5.3 48 ± 3.1
Hg2+ 7 ± 0.5 N.D. N.D.
EDTA 92 ± 7.3 90 ± 5.0 82 ± 2.2
DTT 52 ± 3.5 59 ± 1.8 67 ± 2.3
  1. ±, standard error; ND, not detected