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Table 1 Effect of different metal ions and chemical reagents on MtCh509

From: Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221

Substances

Relative activity (%)

1 mM

5 mM

10 mM

Control

100

100

100

Ni2+

100 ± 5.8

69 ± 6.9

32 ± 1.8

Cu2+

99 ± 5.6

91 ± 4.9

6 ± 1.8

Mg2+

93 ± 5.2

67 ± 6.9

66 ± 3.9

Li+

86 ± 6.3

85 ± 1.6

67 ± 7.4

Ba2+

81 ± 1.0

82 ± 0.4

73 ± 1.3

K+

82 ± 2.8

84 ± 0.9

50 ± 3.4

Zn2+

87 ± 0.8

77 ± 0.0

20 ± 0.6

Mn2+

66 ± 3.2

49 ± 0.9

50 ± 1.1

Ca2+

59 ± 2.5

51 ± 1.6

51 ± 6.0

Co2+

58 ± 2.6

52 ± 1.6

25 ± 2.0

Cs2+

40 ± 2.0

37 ± 5.3

48 ± 3.1

Hg2+

7 ± 0.5

N.D.

N.D.

EDTA

92 ± 7.3

90 ± 5.0

82 ± 2.2

DTT

52 ± 3.5

59 ± 1.8

67 ± 2.3

  1. ±, standard error; ND, not detected