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Fig. 9 | Biotechnology for Biofuels

Fig. 9

From: Expression of an endoglucanase–cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi

Fig. 9

Digestion of PCS with purified L. starkeyi enzymes. Endoglucanase compatibility and cooperativity with different cellobiohydrolase constructs. a TrCBHI, b TeTrCBHI, and c Fusion 3. Each cellobiohydrolase was loaded at the molar equivalent loading of 25 mg of the reference TrCBHI per g of biomass glucan. Endoglucanase (when present) was loaded at the molar equivalent of a loading of A. cellulolyticus E1-CD Y245G mutant at 2 mg/g substrate glucan. A. niger β-glucosidase was loaded in all assays at 0.5 mg/g substrate glucan. Conversion percentages were calculated based on HPLC determinations of released monomeric glucose

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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