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Table 3 Kinetic constants for oxidation of ABTS, DMP, and guaiacol by IlDyP2 and IlDyP1 from I. lacteus

From: Dye-decolorizing peroxidases in Irpex lacteus combining the catalytic properties of heme peroxidases and laccase play important roles in ligninolytic system

Substrate

pH

IlDyP

Km [μM]

kcat [s−1]

kcat/Km [s−1 M−1]

ABTS

3

II

125.0 ± 1.4

396.2 ± 0.0

(3.2 ± 0.0) × 106

4

I

693.1 ± 8.5

356.3 ± 11.2

(5.1 ± 0.1) × 105

DMP

3

II

1631.7 ± 10.9

560.8 ± 19.3

(3.4 ± 0.2) × 105

4

I

892.7 ± 81.5

198.8 ± 12.6

(2.2 ± 0.1) × 105

Guaiacol

3

II

229.1 ± 15.9

49.7 ± 0.9

(2.2 ± 0.1) × 105

4

I

592.1 ± 53.0

49.2 ± 1.5

(8.3 ± 0.5) × 104

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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