Fig. 3

The nucleotide sequence of IS1447. The nucleotides are numbered above the sequence. The putative IR sequences are in purple. The putative initiation and termination codons of the transposase(s) are in green and red, respectively. The italic letters in lower case indicate potential ribosome-binding sites. The green arrows above the sequence indicate the nucleotides from which the cloning sequences for the expression of OrfA, OrfAB, OrfABt, and OrfB proteins (OrfB1, OrfB2, and OrfB3) were selected. The potential slippage-prone region is highlighted in yellow, and the reading frames for OrfA, OrfB, and OrfAB are underlined in black, blue, and red, respectively