Draft genome sequence and detailed characterization of biofuel production by oleaginous microalga Scenedesmus quadricauda LWG002611

Table 2 De novo genome assembly and reference-guided assembly statistics of S. quadricauda LWG002611

Statistics of genome assembly
Estimated genome size 65.35 Mb
De novo assembly		Reference-guided assembly		Misassemblies		Mismatches
Total reads (counts)	57,273,557	Largest alignment (bp)	55,459	Misassemblies	2	Mismatches	127,490
Matched (counts)	53,419,545	Total aligned length (bp)	6,376,645	Relocations	2	Indels	95,642
Mismatched (counts)	3,854,012	Contigs	13,425	Translocations	0	Indels length	102,952
Contigs (counts)	58,317	Contigs (≥ 0 bp)	14,013	Inversions	0	Mismatches per 100 kbp	1982.82
Contig maximum length (bp)	368,038	Contigs (≥ 1000 bp)	11,309	Misassembled contigs	2	Indels per 100 kbp	1487.5
Contig minimum length (bp)	358	Contigs (≥ 5000 bp)	4344	Misassembled contigs length (bp)	12,585	Indels (≤ 5 bp)	95,627
Contig average length (bp)	1704	Contigs (≥ 10,000 bp)	1653	Local misassemblies	216	Indels (> 5 bp)	15
N75	996	Contigs (≥ 25,000 bp)	150	Unaligned miscontigs	2535	N’s	0
N50	2112	Contigs (≥ 50,000 bp)	3			N’s per 100 kbp	0
N25	16,976	Largest contig (bp)	68,181
Total bases	99,381,020	Total length (bp)	65,171,243
GC%	32.5	Total length (≥ 0 bp)	65,393,757
Genes	13,514	Total length (≥ 1000 bp)	63,962,748
		Total length (≥ 5000 bp)	45,404,946
		Total length (≥ 10,000 bp)	26,423,640
		Total length (≥ 25,000 bp)	4,731,985
		Total length (≥ 50,000 bp)	180,197
		N50	8094
		N75	4282
		L50	2350
		L75	5093
		GC (%)	63.2
		Genes	16,739

This assembly and mapping statistics were generated by Quality Assessment Tool for Genome Assemblies’ (QUAST). The de novo assembly and the reference mapping the filtered reads were processed in CLC bio Genomic Workbench 9.0 de novo assembly and reference mapping tool

ISSN: 2731-3654