Fig. 1From: Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesisAcyl-CoA thioesterase activity of ptTES1. a ptTES1 was expressed in frame with lacZ (upper) in E. coli K27 fadD mutant fadD88 and resulted in the color change of E. coli colonies grown on M9 agar plate containing neutral red. Colonies that express an active ptTES1 are dark pink, while those containing empty vector pUC18 or expressing palmitoyl protein thioesterase ptPPT1 (J10454) are light yellow. The time-dependent hydrolysis by ptTES1 of varied concentrations of decanoyl-CoA (10:0), myristoyl-CoA (14:0), and stearoyl-CoA (18:0) (b); palmitoyl-CoA (16:0), oleoyl-CoA (18:1), and eicosapentaenoyl-CoA (20:5) (c); and malonyl-CoA (3:0) (d); was monitored by measuring the change of A420. Reactions were carried out at 37 °C in mixture including 10 mM Hepes (pH 7.5), 50 mM KCl, and 0.3 mM DTNBBack to article page