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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis

Fig. 4

Effect of the ptTES1 gene inactivation on cell growth and lipid accumulation in P. tricornutum. Cell density (a), nitrate concentration in culture media (b), and accumulation of TAGs (c) detected by Nile Red assay (fluorescence intensity normalized to cell number) of wild-type and the ptTES1 knockout mutant (Tes-KO) were analyzed at different days during the growth in F/2-enriched artificial seawater medium. Error bars represent SD of three biological replicates. d Microscopy images (above, wild-type Pt1; below, the ptTES1 knockout mutant Tes-KO) of Bodipy 505/515-stained algal cells grown in F/2 enriched seawater medium with nitrogen deprivation for 72 h

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