Enhanced triacylglycerol production in the diatom Phaeodactylum tricornutum by inactivation of a Hotdog-fold thioesterase gene using TALEN-based targeted mutagenesis

Table 1 Kinetic constants and characteristics of ptTES1-catalyzed acyl-CoA hydrolysis

Substrate	K_m (μM)	V_max (nmol/min/mg)	k_cat (S⁻¹)	k_cat/K_m (M⁻¹ s⁻¹)
Malonyl-CoA	14.64	2.05	0.0009	60.68
Decanoyl-CoA (C10:0)	91.76	22.83	0.0099	107.81
Myristoyl-CoA (C14:0)	21.79	30.25	0.0131	601.58
Palmitoyl-CoA (C16:0)	14.76	45.32	0.0196	1330.53
Stearoyl-CoA (C18:0)	8.246	45.12	0.0196	2371.09
Oleoyl-CoA (C18:1)	12.37	50.75	0.0220	1777.82
Eicosapentaenoyl-CoA (C20:5)	38.61	16.38	0.0071	183.84

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