Fig. 2

Antibiotic sensitivity of W. sorbophila. Cell growth was examined at various concentrations of antibiotics to identify a selectable marker. Cells (1 × 10⁷) were grown for 3 days on yeast peptone dextrose (YPD) agar medium containing blasticidin S, puromycin, Zeocin, hygromycin B, nourseothricin, or phleomycin. For comparison, cell growth is represented by “+” and “−” (+ ≤ 100 colonies; ++ ≤ 200 colonies; +++ ≤ 400 colonies; ++++ > 400 colonies)